About: Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with public DNA sequence databases. Interspecifi c variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for other Asteraceae tribes. All three markers had higher variation (2.1 4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplifi cation was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, especially for very closely related species. The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with public DNA sequence databases. Interspecifi c variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for other Asteraceae tribes. All three markers had higher variation (2.1 4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplifi cation was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, especially for very closely related species. (en)
Title	Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes (en)
skos:prefLabel	Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes (en)
skos:notation	RIV/67985939:_____/12:00381075!RIV13-GA0-67985939
http://linked.open...avai/predkladatel	Botanický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA206/05/0657), P(GAP506/10/1363), Z(AV0Z60050516), Z(MSM0021620828)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Fehrer, Judith Chrtek, Jindřich Krak, Karol Caklová, Petra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Botanický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	130821
http://linked.open...ai/riv/idVysledku	RIV/67985939:_____/12:00381075
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Asteraceae; Hieraciinae; low-copy nuclear markers (en)
http://linked.open.../riv/klicoveSlovo	Asteraceae Hieraciinae low-copy nuclear markers
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[83C5CD363005]
http://linked.open...i/riv/nazevZdroje	American Journal of Botany
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular phylogeny and evolutionary trends in hieracium (Asteraceae, Lactuceae) Phylogeny of subtribe Hieraciinae (Asteraceae) – a model example of contrasting evolutionary strategies in closely related lineages
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	99
http://linked.open...iv/tvurceVysledku	Chrtek, Jindřich Fehrer, Judith Krak, Karol Caklová, Petra Costa, A. Álvarez, I.
http://linked.open...ain/vavai/riv/wos	000304812600011
http://linked.open...n/vavai/riv/zamer	Struktura, funkce a evoluce biodiversity fotoautotrofních organismů a hub: původ a příčiny jejich proměnlivosti, dynamika populací, společenstev a ekosystémů; využití vybraných výsledků k rozvoji Průhonického parku Ekologické procesy v evoluci modelových skupin organismů
issn	0002-9122
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.3732/ajb.1100416
is http://linked.open...avai/riv/vysledek of	Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes

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