About: Characterization of three newly established rat sarcoma cell clones

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Establishment of new animal models using selected cell lines with different behaviour is very important for cancer investigations. In this study, we describe three morphologically distinct rat sarcoma clones-C4, C7 and D6-isolated from the R5-28 cell line. Cells of all clones expressed vimentin, fibronectin, laminin, collagen IV and matrix metalloproteinases 2 and 9. However, desmin, cytokeratins 8 and 18, ZO-1 and desmoplakins I and II were not detected. Significant proliferative capacity was documented by proliferating cell nuclear antigen expression and BrdU positivity. Karyotype of the C4, C7 and D6 cells greatly differed from diploid chromosome number of normal rat somatic cells. High expression of three cytokines-monocyte chemoattractant protein 1, tissue inhibitor of metalloproteinases 1 and vascular endothelial growth factor-was observed in all three clones. However, they varied in concentration of chemokines associated with neutrophil migration and activation-cytokine induced neutrophil chemoattractant 2 and lipopolysaccharide induced CXC chemokine. The C4 clone showed spontaneous tumour regression in vivo that was associated with significant changes in lymphocyte subpopulations. Establishment of new animal models using selected cell lines with different behaviour is very important for cancer investigations. In this study, we describe three morphologically distinct rat sarcoma clones-C4, C7 and D6-isolated from the R5-28 cell line. Cells of all clones expressed vimentin, fibronectin, laminin, collagen IV and matrix metalloproteinases 2 and 9. However, desmin, cytokeratins 8 and 18, ZO-1 and desmoplakins I and II were not detected. Significant proliferative capacity was documented by proliferating cell nuclear antigen expression and BrdU positivity. Karyotype of the C4, C7 and D6 cells greatly differed from diploid chromosome number of normal rat somatic cells. High expression of three cytokines-monocyte chemoattractant protein 1, tissue inhibitor of metalloproteinases 1 and vascular endothelial growth factor-was observed in all three clones. However, they varied in concentration of chemokines associated with neutrophil migration and activation-cytokine induced neutrophil chemoattractant 2 and lipopolysaccharide induced CXC chemokine. The C4 clone showed spontaneous tumour regression in vivo that was associated with significant changes in lymphocyte subpopulations. (en)
Title	Characterization of three newly established rat sarcoma cell clones Characterization of three newly established rat sarcoma cell clones (en)
skos:prefLabel	Characterization of three newly established rat sarcoma cell clones Characterization of three newly established rat sarcoma cell clones (en)
skos:notation	RIV/67985904:_____/12:00386824!RIV13-AV0-67985904
http://linked.open...avai/predkladatel	Ústav živočišné fyziologie a genetiky AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(2B08063), S
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Horák, Vratislav Holubová, Monika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	126851
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/12:00386824
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	sarcoma; cell clones; lewis rat (en)
http://linked.open.../riv/klicoveSlovo	cell clones lewis rat sarcoma
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[4905FA804BD8]
http://linked.open...i/riv/nazevZdroje	In Vitro - Cellular and Developmental Biology. Animal Section
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Research into the effect of a combination of substances for targeted therapy and inhibitory action of the field of pulse vector magnetic potential on oncogenous diseases
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Horák, Vratislav Vannucci, Luca Holubová, Monika Leba, M. Sedmíková, M.
http://linked.open...ain/vavai/riv/wos	000312559100002
issn	1071-2690
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s11626-012-9563-3
is http://linked.open...avai/riv/vysledek of	Characterization of three newly established rat sarcoma cell clones

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