About: Identification of collagen types in tissues using HPLC-MS/MS

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A method for the determination and quantification of collagen types I–V in rat tissues has been developed. This method is based on collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC-MS/MS analyses of the resulting peptide mixtures (peptide maps) were performed.The applicability of this method for analyzing collagen types was demonstrated on rat tissues (skin, tendon, and aorta A method for the determination and quantification of collagen types I–V in rat tissues has been developed. This method is based on collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC-MS/MS analyses of the resulting peptide mixtures (peptide maps) were performed.The applicability of this method for analyzing collagen types was demonstrated on rat tissues (skin, tendon, and aorta (en) Byla vyvinuta metoda pro určení a kvantifikaci kolagenních typů I-V v tkáních potkana. Metoda je založena na fragmentaci kolagenu bromkyanem a štěpení trypsinem. Poté je výsledná peptidová směs analyzována pomocí HPLC-MS/MS. Aplikovatelnost metody byla prokázána při analýze kolagenních typů v tkáních potkana (kůže, šlacha, aorta) (cs)
Title	Identification of collagen types in tissues using HPLC-MS/MS Identification of collagen types in tissues using HPLC-MS/MS (en) Identifikace kolagenních typů v tkáních pomocí HPLC-MS/MS (cs)
skos:prefLabel	Identification of collagen types in tissues using HPLC-MS/MS Identification of collagen types in tissues using HPLC-MS/MS (en) Identifikace kolagenních typů v tkáních pomocí HPLC-MS/MS (cs)
skos:notation	RIV/67985823:_____/08:00315977!RIV09-AV0-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0510), P(GA203/06/1044), P(GA203/08/1428), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika	20
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Mikšík, Ivan Pataridis, Statis Eckhardt, Adam Lacinová, Kateřina Sedláková, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	371210
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/08:00315977
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	collagen; collagen types; proteomics (en)
http://linked.open.../riv/klicoveSlovo	collagen proteomics collagen types
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[35E24AEABB9F]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for cardiovascular research Capillary electrophoresis with multidimensinal detection and its application to separation and characterization of biopolymers Affinity capillary electrophoresis and electrochromatography, their use for study of biomolecular interactions
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Mikšík, Ivan Eckhardt, Adam Pataridis, Statis Sedláková, Pavla Mikulíková, Kateřina
http://linked.open...ain/vavai/riv/wos	261237500001
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka
issn	1615-9306
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Identification of collagen types in tissues using HPLC-MS/MS Identification of collagen types in tissues using HPLC-MS/MS

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