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| rdf:type
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| Description
| - To identify amino acid residues responsible for the determination of the yeast Na+/H+ antiporter substrate specificity (ability to recognize K+), the gene encoding the Zygosaccharomyces rouxii Sod2-22 antiporter (non-transporting K+) was mutagenized and a collection of ZrSod2-22 mutants that improved the KCl tolerance of a salt-sensitive S. cerevisiae strain was isolated. Several independent ZrSod2-22 mutated alleles contained the replacement of highly-conserved proline 145 in the fifth transmembrane domain with a residue containing a hydroxyl group (serine, threonine). The ZrSod2-22p with P145S or P145T substitution mediated, in contrast to the wild-type antiporter, transport of K+. Simultaneously, the P145S(T) mutation decreased the antiporter transport capacity for both Na+ and Li+. Thus, the Pro145 was found to be a critical residue for the yeast plasma membrane antiporters’ function, as far as the substrate specificity range and transport activity are concerned
- To identify amino acid residues responsible for the determination of the yeast Na+/H+ antiporter substrate specificity (ability to recognize K+), the gene encoding the Zygosaccharomyces rouxii Sod2-22 antiporter (non-transporting K+) was mutagenized and a collection of ZrSod2-22 mutants that improved the KCl tolerance of a salt-sensitive S. cerevisiae strain was isolated. Several independent ZrSod2-22 mutated alleles contained the replacement of highly-conserved proline 145 in the fifth transmembrane domain with a residue containing a hydroxyl group (serine, threonine). The ZrSod2-22p with P145S or P145T substitution mediated, in contrast to the wild-type antiporter, transport of K+. Simultaneously, the P145S(T) mutation decreased the antiporter transport capacity for both Na+ and Li+. Thus, the Pro145 was found to be a critical residue for the yeast plasma membrane antiporters’ function, as far as the substrate specificity range and transport activity are concerned (en)
- Aby bylo možné identifikovat aminokyselinové zbytky odpovědné za určení substrátové specifity (rozpoznání K+) u Na+/H+ antiporterů kvasinek, byla provedena mutageneze genu kódujícího antiporter Sod2-22 z kvasinky Zygosaccharomyces rouxii (netransportující K+) a byla izolována kolekce mutantních verzí ZrSod2-22p, jejichž exprese v Saccharomyces cerevisiae (v mutantním kmeni citlivém k solím) vedla ke zvýšené toleranci buněk ke KCl. U několika nezávisle získaných mutantních alel ZrSod2-22 došlo k záměně konzervovaného zbytku prolinu 145 v páté transmembránové doméně za aminokyselinový zbytek obsahující hydroxylovou skupinu (serin, threonin). ZrSod2-22 antiporter obsahující mutaci P145S nebo P145T byl schopen, na rozdíl od nemutovaného přenašeče, transportovat K+. Současně mutace P145S(T) snižovala transportní kapacitu antiporteru pro kationty Na+ a Li+. Pro145 byl tak identifikován jako velmi důležitý zbytek ovlivňující funkci Na+/H+ antiporterů plasmatické membrány kvasinek, co se tý... (cs)
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| Title
| - Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na(+)/H(+) antiporters
- Identifikace konservovaného zbytku prolinu důležitého pro transportní aktivitu a rozsah substrátové specifity Na(+)/H(+) antiporterů plasmatické membrány kvasinek (cs)
- Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na(+)/H(+) antiporters (en)
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| skos:prefLabel
| - Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na(+)/H(+) antiporters
- Identifikace konservovaného zbytku prolinu důležitého pro transportní aktivitu a rozsah substrátové specifity Na(+)/H(+) antiporterů plasmatické membrány kvasinek (cs)
- Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na(+)/H(+) antiporters (en)
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| skos:notation
| - RIV/67985823:_____/05:00001342!RIV06-AV0-67985823
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GP204/02/D092), Z(AV0Z5011922)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/67985823:_____/05:00001342
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - yeast; Na+/H+ antiporter; substrate specificity (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Biological Chemistry
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Sychrová, Hana
- Zavřel, Martin
- Zimmermannová, Olga
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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