About: Activating omega-6 polyunsaturated fatty acids and inhibitory purine nucleotides are high affinity ligands for novel mitochondrial uncoupling proteins UCP2 and UCP3.

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About: Activating omega-6 polyunsaturated fatty acids and inhibitory purine nucleotides are high affinity ligands for novel mitochondrial uncoupling proteins UCP2 and UCP3. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Human UCP2 and UCP3, expressed in yeast, were studied to establish their high affinity regulatory ligands. UCPn were reconstituted into liposomes and assayed for fatty acid (FA)-induced H+ efflux. All natural long chain FAs activated UCP2- and UCP3-mediated H+ translocation. Coenzyme Q10 had no further significant activating effect. Evaluated parameters of FA activation (FA cycling) kinetics revealed the highest apparent affinity to UCP2 for omega-6 polyunsaturated FAs (PUFAs), all-cis-8,11,14-eicosatrienoic and all-cis-6,9,12-octadecatrienoic acids, which are also the most potent agonists of the nuclear PPAR-beta receptor in the activation of UCP2 transcription. Omega-3 PUFA, cis-5,8,11,14,17-eicosapentaenoic acid had lower affinity. Although being omega-6 PUFA, arachidonic acid exhibited the same low affinity as lauric acid. These findings suggest a possible dual role of some PUFAs in activating both, UCPn expression and uncoupling activity. UCP2(UCP3)-dependent H+ translocation activated by all te Human UCP2 and UCP3, expressed in yeast, were studied to establish their high affinity regulatory ligands. UCPn were reconstituted into liposomes and assayed for fatty acid (FA)-induced H+ efflux. All natural long chain FAs activated UCP2- and UCP3-mediated H+ translocation. Coenzyme Q10 had no further significant activating effect. Evaluated parameters of FA activation (FA cycling) kinetics revealed the highest apparent affinity to UCP2 for omega-6 polyunsaturated FAs (PUFAs), all-cis-8,11,14-eicosatrienoic and all-cis-6,9,12-octadecatrienoic acids, which are also the most potent agonists of the nuclear PPAR-beta receptor in the activation of UCP2 transcription. Omega-3 PUFA, cis-5,8,11,14,17-eicosapentaenoic acid had lower affinity. Although being omega-6 PUFA, arachidonic acid exhibited the same low affinity as lauric acid. These findings suggest a possible dual role of some PUFAs in activating both, UCPn expression and uncoupling activity. UCP2(UCP3)-dependent H+ translocation activated by all te (en)
Title	Activating omega-6 polyunsaturated fatty acids and inhibitory purine nucleotides are high affinity ligands for novel mitochondrial uncoupling proteins UCP2 and UCP3. Activating omega-6 polyunsaturated fatty acids and inhibitory purine nucleotides are high affinity ligands for novel mitochondrial uncoupling proteins UCP2 and UCP3. (en)
skos:prefLabel	Activating omega-6 polyunsaturated fatty acids and inhibitory purine nucleotides are high affinity ligands for novel mitochondrial uncoupling proteins UCP2 and UCP3. Activating omega-6 polyunsaturated fatty acids and inhibitory purine nucleotides are high affinity ligands for novel mitochondrial uncoupling proteins UCP2 and UCP3. (en)
skos:notation	RIV/67985823:_____/03:20030100!RIV/2004/MSM/A20004/N
http://linked.open.../vavai/riv/strany	20761;20769
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/02/1215), P(IAA5011106), P(ME 389), Z(AV0Z5011922)
http://linked.open...iv/cisloPeriodika	23
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Urbánková, Eva Škobisová, Eva Ježek, Petr Žáčková, Markéta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	597369
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/03:20030100
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	uncoupling protein-2; polyunsaturated fatty acids; recombinant yeast expression (en)
http://linked.open.../riv/klicoveSlovo	polyunsaturated fatty acids recombinant yeast expression uncoupling protein-2
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C1EAE5FD2A87]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterization of mRNA variations for novel mitochondrial uncoupling proteins during stimulations by pyrogenic cytokines, thyroid hormones and lipoid compound Studies of novel mitochondrial uncoupling proteins http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20389
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	278
http://linked.open...iv/tvurceVysledku	Ježek, Petr Žáčková, Markéta Urbánková, Eva Škobisová, Eva
http://linked.open...n/vavai/riv/zamer	Investigation of normal and pathological physiology of animals - the clarification of the physiological mechanisms operating in humans in health and disease
issn	0021-9258
number of pages	9 (xsd:int)

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