About: Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport.

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mutants were constructed for mitochondrial uncoupling protein UCP1, with single or multiple substitutions within or nearby the UCP-signatures located in the 1st alfa-helix and 2nd matrix segment, using the QuickChange site directed mutagenesis protocol (Stratagene), and were assayed fluorometrically for kinetics of fatty acid (FA)-induced H+ uniport and for Cl- uniport. Their ability to bind 3H-GTP was also evaluated. The wild type UCP1 was associated with the FA-induced H+ uniport proportional to the added protein with a Km for lauric acid of 43 mikroM and Vmax of 18 mikromol.min-1.(mg protein)-1. Neutralization of Arg 152 (in the 2nd matrix segment UCP-signature) led to ~50% reduction of FA affinity (reciprocal Km) and of Vmax for FA-induced H+ uniport. Halved FA-affinity and 70% reduction of Vmax was found for the double His substitution outside the signature (H145L & H147L mutant). Neutralization of Asp 27 in the1st alfa-helix UCP-signature (D27V mutant) resulted in 75% reduction of FA affinity an Mutants were constructed for mitochondrial uncoupling protein UCP1, with single or multiple substitutions within or nearby the UCP-signatures located in the 1st alfa-helix and 2nd matrix segment, using the QuickChange site directed mutagenesis protocol (Stratagene), and were assayed fluorometrically for kinetics of fatty acid (FA)-induced H+ uniport and for Cl- uniport. Their ability to bind 3H-GTP was also evaluated. The wild type UCP1 was associated with the FA-induced H+ uniport proportional to the added protein with a Km for lauric acid of 43 mikroM and Vmax of 18 mikromol.min-1.(mg protein)-1. Neutralization of Arg 152 (in the 2nd matrix segment UCP-signature) led to ~50% reduction of FA affinity (reciprocal Km) and of Vmax for FA-induced H+ uniport. Halved FA-affinity and 70% reduction of Vmax was found for the double His substitution outside the signature (H145L & H147L mutant). Neutralization of Asp 27 in the1st alfa-helix UCP-signature (D27V mutant) resulted in 75% reduction of FA affinity an (en)
Title	Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport. Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport. (en)
skos:prefLabel	Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport. Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport. (en)
skos:notation	RIV/67985823:_____/03:20030011!RIV/2004/GA0/A20004/N
http://linked.open.../vavai/riv/strany	212;220
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/02/1215), P(GA301/98/0568), P(IAA5011106), Z(AV0Z5011922)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Urbánková, Eva Škobisová, Eva Ježek, Petr Hanák, Petr Růžička, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	629679
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/03:20030011
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	site-directed mutagenesis; mitochondrial uncoupling protein-1; fatty acid-inducted H+ uniport (en)
http://linked.open.../riv/klicoveSlovo	site-directed mutagenesis fatty acid-inducted H+ uniport mitochondrial uncoupling protein-1
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[DD294738C8DC]
http://linked.open...i/riv/nazevZdroje	International Journal of Biochemistry & Cell Biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterization of mRNA variations for novel mitochondrial uncoupling proteins during stimulations by pyrogenic cytokines, thyroid hormones and lipoid compound Molecular biology and structure funcition studies of multiple substitution mutants of mitochondrial uncoupling protein Studies of novel mitochondrial uncoupling proteins
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	35
http://linked.open...iv/tvurceVysledku	Ježek, Petr Růžička, Michal Hanák, Petr Urbánková, Eva Škobisová, Eva
http://linked.open...n/vavai/riv/zamer	Investigation of normal and pathological physiology of animals - the clarification of the physiological mechanisms operating in humans in health and disease
issn	1357-2725
number of pages	9 (xsd:int)

Faceted Search & Find service v1.16.116 as of Feb 22 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3239 as of Feb 22 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 81 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software