About: A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that signiÞcantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were veriÞed by testing each sample. A simulation study was used to compare the efÞciency of the new method with testing all the samples and to develop a decision tool for determining when best to use the new method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less). American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that signiÞcantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were veriÞed by testing each sample. A simulation study was used to compare the efÞciency of the new method with testing all the samples and to develop a decision tool for determining when best to use the new method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less). (en)
Title	A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris (en)
skos:prefLabel	A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris (en)
skos:notation	RIV/67179843:_____/12:00384289!RIV13-AV0-67179843
http://linked.open...avai/predkladatel	Centrum výzkumu globální změny AV ČR v.v.i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(SP/2D3/60/08), Z(AV0Z60870520), Z(MSM0021620828)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kindlmann, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	120373
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/12:00384289
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	polymerase chain reaction; matrix; honey bee; Apis mellifera; AFB (en)
http://linked.open.../riv/klicoveSlovo	polymerase chain reaction AFB honey bee matrix Apis mellifera
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C0BCA6DFD774]
http://linked.open...i/riv/nazevZdroje	Journal of Economic Entomology
http://linked.open...in/vavai/riv/obor	EH
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated research of the effects and consequences of artificially reared mallards (Anas platyrhynchos) on wetland ecosystems with the application of advanced ecological and molecular techniques.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	105
http://linked.open...iv/tvurceVysledku	Kindlmann, Pavel Stopka, P. Haklová, M. Ryba, Š. Titěra, D.
http://linked.open...ain/vavai/riv/wos	000312443000002
http://linked.open...n/vavai/riv/zamer	Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Ekologické procesy v evoluci modelových skupin organismů
issn	0022-0493
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris

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