About: Cattle breed discrimination based on microsatellites markers

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Description	The aim of this study was utilize microsatellites for routine parentage testing and identification in breed cattle discrimination. The analysis was performed in three beef cattle (Aberdeen Angus n= 93, Hereford n= 125, Charolais n = 101) and one dairy cattle breed (Czech Fleckvieh n = 46). In total, 365 animals were genotyped with using Finnish Bovine Genotypes Panel 3.1 for 18 microsatellite markers recommended by both ISAG for parentage testing and FAO for genetic studies of domestic animals (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824, BM1818, and SPS113, RM067, CSRM60, MGTG4B, CSSM66, ILSTS006, respectively). The STRUCTURE (version 2.2) program based on a Bayesian clustering method was used to obtain population structure. The software detected four clusters corresponding to the four breeds without using prior information. The genetic markers used in study were suitable for good clustering ability by evidencing good discrimination power. Differentiation among individuals of each breed characterized by Fst values range from the lowest 0.091 in Aberdeen Angus, 0.099 in Czech Fleckvieh, 0.122 in Charolais, to highest 0.146 in Hereford cattle population. Average distances (expected heterozygosity) between individuals in same cluster were for Czech Fleckvieh 0.684, Charolais 0.702, Aberdeen Angus 0.722, and Hereford 0.683. Research data certified the possibility of using the Finnish Bovine Genotypes Panel 3.1 of DNA microsatellites markers for the cattle breed discrimination. The aim of this study was utilize microsatellites for routine parentage testing and identification in breed cattle discrimination. The analysis was performed in three beef cattle (Aberdeen Angus n= 93, Hereford n= 125, Charolais n = 101) and one dairy cattle breed (Czech Fleckvieh n = 46). In total, 365 animals were genotyped with using Finnish Bovine Genotypes Panel 3.1 for 18 microsatellite markers recommended by both ISAG for parentage testing and FAO for genetic studies of domestic animals (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824, BM1818, and SPS113, RM067, CSRM60, MGTG4B, CSSM66, ILSTS006, respectively). The STRUCTURE (version 2.2) program based on a Bayesian clustering method was used to obtain population structure. The software detected four clusters corresponding to the four breeds without using prior information. The genetic markers used in study were suitable for good clustering ability by evidencing good discrimination power. Differentiation among individuals of each breed characterized by Fst values range from the lowest 0.091 in Aberdeen Angus, 0.099 in Czech Fleckvieh, 0.122 in Charolais, to highest 0.146 in Hereford cattle population. Average distances (expected heterozygosity) between individuals in same cluster were for Czech Fleckvieh 0.684, Charolais 0.702, Aberdeen Angus 0.722, and Hereford 0.683. Research data certified the possibility of using the Finnish Bovine Genotypes Panel 3.1 of DNA microsatellites markers for the cattle breed discrimination. (en)
Title	Cattle breed discrimination based on microsatellites markers Cattle breed discrimination based on microsatellites markers (en)
skos:prefLabel	Cattle breed discrimination based on microsatellites markers Cattle breed discrimination based on microsatellites markers (en)
skos:notation	RIV/62156489:43210/11:00181026!RIV12-MSM-43210___
http://linked.open...avai/predkladatel	Agronomická fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B08037)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Vrtková, Irena Stehlík, Libor Bartoňová, Petra Putnová, Lenka
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	189309
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/11:00181026
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	discrimination; cattle; microsatellite (en)
http://linked.open.../riv/klicoveSlovo	cattle discrimination microsatellite
http://linked.open...ontrolniKodProRIV	[6F25F4B16E98]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnological methods for innovations of manufacturing and consumerist quality evaluation of beef as source of an animal proteins.
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Bartoňová, Petra Putnová, Lenka Stehlík, Libor Vrtková, Irena
http://localhost/t...ganizacniJednotka	43210

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