About: A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element

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About: A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/elps.201300079/full
Description	The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA-protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ??P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences. The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA-protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ??P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences. (en)
Title	A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element (en)
skos:prefLabel	A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element (en)
skos:notation	RIV/61989592:15310/13:33147707!RIV14-GA0-15310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(GA13-01809S), P(GAP303/12/0472), S
http://linked.open...iv/cisloPeriodika	13
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Vrzal, Radim Dvořák, Zdeněk Dořičáková, Aneta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	58870
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/13:33147707
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Response elements; Pregnane X receptor; Electrophoretic mobility shift assay; Drug metabolism; Cytochrome P450 (en)
http://linked.open.../riv/klicoveSlovo	Electrophoretic mobility shift assay Response elements Cytochrome P450 Drug metabolism Pregnane X receptor
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[D7AB5E358DB5]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Enantiospecifické interakce mezi klinicky užívanými chirálními léčivy a regulačními drahami humánních cytochromů P450. Novel mechanisms of CYP3A4 enzyme regulation based on posttranslational modification of nuclear receptors - implications for pharmacotherapy
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Dvořák, Zdeněk Vrzal, Radim Vávrová, Aneta
http://linked.open...ain/vavai/riv/wos	000322112200003
issn	0173-0835
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/elps.201300079
http://localhost/t...ganizacniJednotka	15310

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