About: Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts

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About: Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This paper reports on haploid and mixoploid protoplasts in the genus Cucumis. The mixoploid plants were obtained after oryzalin treatments and the mesophyll protoplasts were isolated and cultuvated. The isolation and cultivation of the cucumber and muskmelon haploid protoplasts from young-stage pollen grains were improved. Somatic hybridization (fusion) between mixoploid cucumber protoplasts and muskmelon mesophyll and callus protoplasts by chemical fusion with polyethylene glycol (PEG, 6000) was performed for the first time. Heterofusants were observed and developed into microcolonies. Gametosomatic hybridization between mixoploid cucumber protoplasts and pollen muskmelon protoplasts was performed for the first time. heterofusants and first cell division were observed. Different ploidy, especially the mixoploid character of isolated protoplasts, has positive influence on protoplasts isolation and the following fusion. This paper reports on haploid and mixoploid protoplasts in the genus Cucumis. The mixoploid plants were obtained after oryzalin treatments and the mesophyll protoplasts were isolated and cultuvated. The isolation and cultivation of the cucumber and muskmelon haploid protoplasts from young-stage pollen grains were improved. Somatic hybridization (fusion) between mixoploid cucumber protoplasts and muskmelon mesophyll and callus protoplasts by chemical fusion with polyethylene glycol (PEG, 6000) was performed for the first time. Heterofusants were observed and developed into microcolonies. Gametosomatic hybridization between mixoploid cucumber protoplasts and pollen muskmelon protoplasts was performed for the first time. heterofusants and first cell division were observed. Different ploidy, especially the mixoploid character of isolated protoplasts, has positive influence on protoplasts isolation and the following fusion. (en)
Title	Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion (en)
skos:prefLabel	Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion (en)
skos:notation	RIV/61989592:15310/12:33140482!RIV13-MSM-15310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika	85
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Navrátilová, Božena Vašut, Radim Jan Skálová, Dagmar Lebeda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	138626
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/12:33140482
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cucumber, muskmelon, mixoploid, polyploidization, protoplast isolation and fusion (en)
http://linked.open.../riv/klicoveSlovo	polyploidization cucumber mixoploid muskmelon protoplast isolation and fusion
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[787593F0C70A]
http://linked.open...i/riv/nazevZdroje	Journal of Applied Botany and Food Quality-Angewandte Botanik
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	85
http://linked.open...iv/tvurceVysledku	Lebeda, Aleš Navrátilová, Božena Skálová, Dagmar Vašut, Radim Jan Doležalová, Ivana
http://linked.open...ain/vavai/riv/wos	000310189700009
http://linked.open...n/vavai/riv/zamer	Variabilita složek a interakcí v rostlinném patosystému a vliv faktorů prostředí na jejich projev
issn	1613-9216
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion

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