About: Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We investigated the effectiveness of short mitochondrial DNA fragments for the identification of lycid larvae. The rrnL, cox1 and nad5 mtDNA sequences from 17 specimens of immature stages of Lycidae and Lampyridae were combined with a previously published dataset of homologous fragments representing all major lineages of Lycidae and outgroups. Their relationships were analysed under parsimony criteria. We demonstrate that high-density profiles are necessary for accurate identification of unknown samples to generic and tribal levels and that a multilocus approach is critical for obtaining reliable results. Although widely used, the cox1 mtDNA fragment showed the worst performance for identification at genus level when the query species was not present in the library. Stronger support for deeper branches came from rrnL mtDNA. The neotenic female larvae and male adult stages of Platerodrilus sp. and Macrolibnetis depressus Pic, 1938 were associated by mtDNA fragments. Based on the present identification, We investigated the effectiveness of short mitochondrial DNA fragments for the identification of lycid larvae. The rrnL, cox1 and nad5 mtDNA sequences from 17 specimens of immature stages of Lycidae and Lampyridae were combined with a previously published dataset of homologous fragments representing all major lineages of Lycidae and outgroups. Their relationships were analysed under parsimony criteria. We demonstrate that high-density profiles are necessary for accurate identification of unknown samples to generic and tribal levels and that a multilocus approach is critical for obtaining reliable results. Although widely used, the cox1 mtDNA fragment showed the worst performance for identification at genus level when the query species was not present in the library. Stronger support for deeper branches came from rrnL mtDNA. The neotenic female larvae and male adult stages of Platerodrilus sp. and Macrolibnetis depressus Pic, 1938 were associated by mtDNA fragments. Based on the present identification, (en)
Title	Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility (en)
skos:prefLabel	Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility (en)
skos:notation	RIV/61989592:15310/09:00010605!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/06/1392), Z(MSM6198959212)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Levkaničová, Zuzana Bocák, Ladislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	318355
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010605
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Coleoptera; Lycidae; larvae; mtDNA; identification (en)
http://linked.open.../riv/klicoveSlovo	larvae Coleoptera identification mtDNA Lycidae
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[60741E6BA4BB]
http://linked.open...i/riv/nazevZdroje	Systematic Entomology
http://linked.open...in/vavai/riv/obor	EG
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Neoteny and speciation in elateroid beetles
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Bocák, Ladislav Levkaničová, Zuzana
http://linked.open...n/vavai/riv/zamer	Life-history strategies of animals: proximate and ultimate mechanisms
issn	0307-6970
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility

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