About: Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS.

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Abstract We have developed an effective method for the isolation, identification, and quantification of several mammalian steroid hormones and their metabolites in different plant tissues. The purification protocol was based on solid-phase extraction (SPE) combined with immunoaffinity chromatography (IAC) using immobilized generic polyclonal anti-D4-3-keto-steroid antibodies covalently bound to Affi-Gel 10 sorbent. The antibodies were characterized by means of enzyme-linked immunosorbent assay (ELISA). The detection limit of the ELISA was 6.0x10e-10 mol/L and cross-reactivity with most D4-3-keto-steroids was very high as predicted (68?122%). The IAC allowed fast, single-step purification of different plant extracts prior to analysis by ultra-performance liquid chromatography-electrospray tandem mass spektrometry [UPLC-ESI(+)-MS/MS]. In multiple-reaction-monitoring (MRM) mode, the detection limit of the method for most of the steroids analyzed was close to 10 fmol and the response was linear up to 50 p Abstract We have developed an effective method for the isolation, identification, and quantification of several mammalian steroid hormones and their metabolites in different plant tissues. The purification protocol was based on solid-phase extraction (SPE) combined with immunoaffinity chromatography (IAC) using immobilized generic polyclonal anti-D4-3-keto-steroid antibodies covalently bound to Affi-Gel 10 sorbent. The antibodies were characterized by means of enzyme-linked immunosorbent assay (ELISA). The detection limit of the ELISA was 6.0x10e-10 mol/L and cross-reactivity with most D4-3-keto-steroids was very high as predicted (68?122%). The IAC allowed fast, single-step purification of different plant extracts prior to analysis by ultra-performance liquid chromatography-electrospray tandem mass spektrometry [UPLC-ESI(+)-MS/MS]. In multiple-reaction-monitoring (MRM) mode, the detection limit of the method for most of the steroids analyzed was close to 10 fmol and the response was linear up to 50 p (en)
Title	Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS. Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS. (en)
skos:prefLabel	Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS. Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS. (en)
skos:notation	RIV/61989592:15310/09:00010048!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(KAN200380801), S, Z(AV0Z40550506), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Novák, Ondřej Strnad, Miroslav Simerský, Radim
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	318316
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010048
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ultra-performance liquid chromatography (UPLC); tandem mass spectrometry (MS/MS); immunoaffinity purification; steroids; plant extracts; Digitalis purpurea; Nicotiana tabacum; Inula helenium (en)
http://linked.open.../riv/klicoveSlovo	steroids plant extracts Ultra-performance liquid chromatography (UPLC) tandem mass spectrometry (MS/MS) Nicotiana tabacum Digitalis purpurea Inula helenium immunoaffinity purification
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[F0B03E9E25CB]
http://linked.open...i/riv/nazevZdroje	Journal of Plant Growth Regulation
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Immunonanotechnologies for hormone diagnostic
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Novák, Ondřej Pouzar, Vladimír Strnad, Miroslav Simerský, Radim Morris, David Arthur
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0721-7595
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS. Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS. Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS. Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS.

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