About: Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization

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About: Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Visual inspections of elm trees in south Moravia in 1997-2007 revealed a rare occurrence of plants with smaller and cowl-forming leaves on some twigs, i.e. a feature resembling witches-broom disease observed on the end of twigs. The presence of phytoplasma-like bodies was observed by transmission electron microscopy of phloem tissue. On the other hand, no phytoplasmas were found in asymptomatic trees. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rRNA sequences specific for phytoplasmas. Sequence analyses of the 16S?23S ribosomal operon (1852 bp) allowed for the classification of the detected phytoplasmas in the elm yellows group, but its position remained on the boundary of the 16SrV-A and 16SrV-C ribosomal subgroups. Sequence analyses of the ribosomal protein of the rpl22-rps3 and secY genes lead to further classification and revealed the phytoplasmas? affiliations to the ?Candidates Phytoplasma ulmi?. Some exceptions in unique oligonucleotide s Visual inspections of elm trees in south Moravia in 1997-2007 revealed a rare occurrence of plants with smaller and cowl-forming leaves on some twigs, i.e. a feature resembling witches-broom disease observed on the end of twigs. The presence of phytoplasma-like bodies was observed by transmission electron microscopy of phloem tissue. On the other hand, no phytoplasmas were found in asymptomatic trees. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rRNA sequences specific for phytoplasmas. Sequence analyses of the 16S?23S ribosomal operon (1852 bp) allowed for the classification of the detected phytoplasmas in the elm yellows group, but its position remained on the boundary of the 16SrV-A and 16SrV-C ribosomal subgroups. Sequence analyses of the ribosomal protein of the rpl22-rps3 and secY genes lead to further classification and revealed the phytoplasmas? affiliations to the ?Candidates Phytoplasma ulmi?. Some exceptions in unique oligonucleotide s (en)
Title	Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization (en)
skos:prefLabel	Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization (en)
skos:notation	RIV/61989592:15310/09:00010040!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Navrátil, Milan Šafářová, Dana Válová, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	333443
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010040
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	POLYMERASE CHAIN-REACTION; MYCOPLASMALIKE ORGANISMS; ELM YELLOWS; GENETIC RELATEDNESS; PCR AMPLIFICATION; NONRIBOSOMAL DNA; PHLOEM NECROSIS; 16S RDNA; CLASSIFICATION; DIFFERENTIATION (en)
http://linked.open.../riv/klicoveSlovo	16S RDNA ELM YELLOWS GENETIC RELATEDNESS MYCOPLASMALIKE ORGANISMS NONRIBOSOMAL DNA PCR AMPLIFICATION PHLOEM NECROSIS POLYMERASE CHAIN-REACTION CLASSIFICATION DIFFERENTIATION
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[FC9F614C6C10]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Navrátil, Milan Šafářová, Dana Fránová, Jana Válová, Pavla Šimková, Miroslava
http://linked.open...n/vavai/riv/zamer	Variabilita složek a interakcí v rostlinném patosystému a vliv faktorů prostředí na jejich projev
issn	0015-5632
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization Phytoplasma associated with witches-broom disease of Ulmus minor Mill . in the Czech Republic: Electron microscopy and molecular characterization

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