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Description
| - V práci bylo zjištěno, že v ranném stádiu působeni UV-B produkce reaktivních forem kyslíku (hydroxalové radikály a peroxid vodíku) závisí na elektronovém transportu a ne výhradně na UV-B. (cs)
- The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (-OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation of terephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm/-2), -OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of -OH was still observed in thylakoids, even though no free hydrogen peroxide was detected.
- The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (-OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation of terephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm/-2), -OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of -OH was still observed in thylakoids, even though no free hydrogen peroxide was detected. (en)
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Title
| - Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.
- Hydroxylové radikály nejsou protagonisté poškození izolovaných thylakoidních membrán pomocí UV-B. (cs)
- Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes. (en)
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skos:prefLabel
| - Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.
- Hydroxylové radikály nejsou protagonisté poškození izolovaných thylakoidních membrán pomocí UV-B. (cs)
- Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes. (en)
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skos:notation
| - RIV/61989592:15310/07:00004841!RIV08-MSM-15310___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/07:00004841
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - hydroxal radicals; UV-B; thylakoid membranes; terephtalate (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - AU - Australské společenství
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Šnyrychová, Iva
- Hideg, Eva
- Kós, Péter
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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