About: Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.     Goto   Sponge   NotDistinct   Permalink

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Description
  • V práci bylo zjištěno, že v ranném stádiu působeni UV-B produkce reaktivních forem kyslíku (hydroxalové radikály a peroxid vodíku) závisí na elektronovém transportu a ne výhradně na UV-B. (cs)
  • The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (-OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation of terephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm/-2), -OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of -OH was still observed in thylakoids, even though no free hydrogen peroxide was detected.
  • The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (-OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation of terephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm/-2), -OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of -OH was still observed in thylakoids, even though no free hydrogen peroxide was detected. (en)
Title
  • Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.
  • Hydroxylové radikály nejsou protagonisté poškození izolovaných thylakoidních membrán pomocí UV-B. (cs)
  • Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes. (en)
skos:prefLabel
  • Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.
  • Hydroxylové radikály nejsou protagonisté poškození izolovaných thylakoidních membrán pomocí UV-B. (cs)
  • Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes. (en)
skos:notation
  • RIV/61989592:15310/07:00004841!RIV08-MSM-15310___
http://linked.open.../vavai/riv/strany
  • 1112-1121
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika
  • 12
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 425143
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/07:00004841
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • hydroxal radicals; UV-B; thylakoid membranes; terephtalate (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • AU - Australské společenství
http://linked.open...ontrolniKodProRIV
  • [F5BBC807CF72]
http://linked.open...i/riv/nazevZdroje
  • Functional Plant Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 34
http://linked.open...iv/tvurceVysledku
  • Šnyrychová, Iva
  • Hideg, Eva
  • Kós, Péter
http://linked.open...n/vavai/riv/zamer
issn
  • 1445-4408
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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