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rdf:type
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Description
| - Borate buffers are common background electrolytes for analyses of anions in capillary zone electrophoresis. Usually, sodium borate at a given pH is used and this specification seems to be sufficient for a successful analysis. In this paper, we show that free migration of OH- may deteriorate the analysis of a typical anionic analysis of clinical samples due to uncontrolled migration of OH- throughout the systems of analyzed zones and may damage the stacking of anionic analytes of interest. We have proven that the use of ammonium borate may remedy the situation where the presence of ammonium may selectively stop the free migration of OH- ions, slow down their effective mobility and bring their safe behavior resulting in reproducible stacking of clinically important anions. Results of real analyses of human serum samples confirmed the proposed method and proved that substitution of sodium for ammonium in borate buffers offers reliable analyses of clinical samples having chloride as the bulk component. Th
- Borate buffers are common background electrolytes for analyses of anions in capillary zone electrophoresis. Usually, sodium borate at a given pH is used and this specification seems to be sufficient for a successful analysis. In this paper, we show that free migration of OH- may deteriorate the analysis of a typical anionic analysis of clinical samples due to uncontrolled migration of OH- throughout the systems of analyzed zones and may damage the stacking of anionic analytes of interest. We have proven that the use of ammonium borate may remedy the situation where the presence of ammonium may selectively stop the free migration of OH- ions, slow down their effective mobility and bring their safe behavior resulting in reproducible stacking of clinically important anions. Results of real analyses of human serum samples confirmed the proposed method and proved that substitution of sodium for ammonium in borate buffers offers reliable analyses of clinical samples having chloride as the bulk component. Th (en)
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Title
| - Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis
- Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis (en)
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skos:prefLabel
| - Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis
- Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis (en)
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skos:notation
| - RIV/61989592:15310/04:00005826!RIV10-MSM-15310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA203/02/0023), P(IAA4031401), S, Z(AV0Z4031919)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/04:00005826
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - background electrolyte; borate buffer, capillary zone electrophoresis; serum analysis; stacking; transient isotachophoresis (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Boček, Petr
- Křivánková, Ludmila
- Gebauer, Petr
- Mrázková, Michaela
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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