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| Description
| - Objectives The aim of this study was to test the survival of microorganisms in eluents used for Ge-68/Ga-68 generators and for regeneration of nonradioactive Ge-68/Ga-68 generator columns after high microbial load. Nonradioactive generator columns were loaded with various microorganisms and tested to determine whether the microorganisms were proliferating or surviving in eluates of the columns. Materials and methods TiO2 columns used in a commercially available Ge-68/Ga-68 generators for absorption of Ge-68 were loaded with greater than 10(7) colony-forming units (CFU) of Staphylococcus aureus, Clostridium sporogenes, Candida albicans, Helicobacter pylori, Aspergillus niger and Deinococcus radiodurans. Columns were eluted with 0.1 N HCl under standard eluting conditions. Elutions, over a prolonged time period, were tested for endotoxins, sterility and number of CFU. Results Initial tests on the eluent (0.1 N HCl) already showed limited survival of microorganisms. Using TiO2 generator columns, even with a higher load of microorganisms no survival of any microorganism could be detected in subsequent elutions, neither in colony-forming unit assays nor in sterility testing. In addition, endotoxin tests showed no elevated levels in any of the eluent samples, even days after incubation. Conclusion The conditions used in this type of Ge-68/Ga-68 generator are highly unfavourable for survival or growth of microorganisms. The risks associated with incidental microbial contaminations during the lifetime of such a generator are therefore very low. Nucl Med Commun 33:819-823 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
- Objectives The aim of this study was to test the survival of microorganisms in eluents used for Ge-68/Ga-68 generators and for regeneration of nonradioactive Ge-68/Ga-68 generator columns after high microbial load. Nonradioactive generator columns were loaded with various microorganisms and tested to determine whether the microorganisms were proliferating or surviving in eluates of the columns. Materials and methods TiO2 columns used in a commercially available Ge-68/Ga-68 generators for absorption of Ge-68 were loaded with greater than 10(7) colony-forming units (CFU) of Staphylococcus aureus, Clostridium sporogenes, Candida albicans, Helicobacter pylori, Aspergillus niger and Deinococcus radiodurans. Columns were eluted with 0.1 N HCl under standard eluting conditions. Elutions, over a prolonged time period, were tested for endotoxins, sterility and number of CFU. Results Initial tests on the eluent (0.1 N HCl) already showed limited survival of microorganisms. Using TiO2 generator columns, even with a higher load of microorganisms no survival of any microorganism could be detected in subsequent elutions, neither in colony-forming unit assays nor in sterility testing. In addition, endotoxin tests showed no elevated levels in any of the eluent samples, even days after incubation. Conclusion The conditions used in this type of Ge-68/Ga-68 generator are highly unfavourable for survival or growth of microorganisms. The risks associated with incidental microbial contaminations during the lifetime of such a generator are therefore very low. Nucl Med Commun 33:819-823 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins. (en)
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| Title
| - Microbial challenge tests on nonradioactive TiO2-based 68Ge/68Ga generator columns
- Microbial challenge tests on nonradioactive TiO2-based 68Ge/68Ga generator columns (en)
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| skos:prefLabel
| - Microbial challenge tests on nonradioactive TiO2-based 68Ge/68Ga generator columns
- Microbial challenge tests on nonradioactive TiO2-based 68Ge/68Ga generator columns (en)
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| skos:notation
| - RIV/61989592:15110/12:33140503!RIV13-MSM-15110___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/12:33140503
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - microorganisms; generator column eluate; Ge-68/Ga-68 generator (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Nuclear Medicine Communications
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Becker, Dirk
- Decristoforo, Clemens
- Lass-Flörl, Cornelia
- Perkhofer, Susanne
- Petřík, Miloš
- Schuessele, Andrea
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1097/MNM.0b013e3283543323
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| http://localhost/t...ganizacniJednotka
| |
| is http://linked.open...avai/riv/vysledek
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