About: HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells

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About: HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells Goto Sponge NotDistinct Permalink

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Description	We examined whether histone deacetylase (HDAC) inhibitors butyrate and valproate alter expression of NCOR1 and NCOR2 genes coding for N-CoR and SMRT, respectively. Leukemic HL-60 cells were treated for 24 h with 0.5 and 1 mM butyrate, 1-3 mM valproate, 1 ?M retinoic acid (RA) or cotreated with 1 ?M RA and 0.5 mM butyrate. Histone acetylation was analysed by western blotting. Levels of NCOR1 and NCOR2 mRNA were determined by real-time PCR. Expression of NCF2 gene coding for p67phox was evaluated as myeloid differentiation marker. Butyrate and valproate increased acetylation of histones H3 and H4. They also increased NCF2 mRNA without affecting NCOR1 or NCOR2 mRNA levels. Similarly, RA alone or in combination with butyrate induced NCF2 gene expression without affecting NCOR1 or NCOR2 mRNA levels. We conclude that HDAC inhibitors do not alter expression of NCOR1 or NCOR2 genes and that the onset of myeloid differentiation is not accompanied by induction or repression of these genes in HL-60 cells. We examined whether histone deacetylase (HDAC) inhibitors butyrate and valproate alter expression of NCOR1 and NCOR2 genes coding for N-CoR and SMRT, respectively. Leukemic HL-60 cells were treated for 24 h with 0.5 and 1 mM butyrate, 1-3 mM valproate, 1 ?M retinoic acid (RA) or cotreated with 1 ?M RA and 0.5 mM butyrate. Histone acetylation was analysed by western blotting. Levels of NCOR1 and NCOR2 mRNA were determined by real-time PCR. Expression of NCF2 gene coding for p67phox was evaluated as myeloid differentiation marker. Butyrate and valproate increased acetylation of histones H3 and H4. They also increased NCF2 mRNA without affecting NCOR1 or NCOR2 mRNA levels. Similarly, RA alone or in combination with butyrate induced NCF2 gene expression without affecting NCOR1 or NCOR2 mRNA levels. We conclude that HDAC inhibitors do not alter expression of NCOR1 or NCOR2 genes and that the onset of myeloid differentiation is not accompanied by induction or repression of these genes in HL-60 cells. (en)
Title	HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells (en)
skos:prefLabel	HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells (en)
skos:notation	RIV/61989592:15110/11:10223831!RIV12-MSM-15110___
http://linked.open...avai/predkladatel	Lékařská fakulta
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0030/01/01), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Ulrichová, Jitka Trtková, Kateřina Vrba, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	201777
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/11:10223831
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Differentiation; Gene expression; All-trans retinoic acid; Valproic acid; NADPH oxidase; Histone deacetylase; Nuclear corepressor (en)
http://linked.open.../riv/klicoveSlovo	Gene expression Differentiation All-trans retinoic acid Histone deacetylase NADPH oxidase Nuclear corepressor Valproic acid
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[FD3AD3871CB7]
http://linked.open...i/riv/nazevZdroje	Biomedical Papers-Olomouc
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomedicine for regional development and human resources
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	155
http://linked.open...iv/tvurceVysledku	Ulrichová, Jitka Trtková, Kateřina Vrba, Jiří
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1213-8118
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	15110
is http://linked.open...avai/riv/vysledek of	HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells

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