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| rdf:type
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| Description
| - V této práci ukazujeme, že barviva kumarin 6, hypericin, ethylresorufin a resorufin jsou vhodné k fluorescenční spektroskopiii a dokazujeme jejich použití ve fiziologicky významných studiích proteinu. (cs)
- In this work we show that the dyes coumarin 6, hypericin, 7-O-ethylresorufin and resorufin are suitable for fluorescence correlation spectroscopy (FCS) and demonstrate the use of these dyes in physiologically relevant protein studies. Since coumarins are metabolised by cytochromes P450, the binding of coumarin 6 to cytochrome P450 3A4 was investigated by FCS. Coumarin 6 appears to be a very bright non-covalent cytochrome P450 label. When titrating cytochrome P450 3A4 with coumarin 6, the diffusion time of the coumarin 6/ cytochrome P450 3A4 complex increases roughly two-fold at protein concentrations higher than 1 μmol l<sup>-1</sup>, indicating the formation of cytochrome aggregates. FCS of the flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) shows that both endogenous dyes undergo photobleaching. Moreover, FAD appears to be present to great extent, as a non-fluorescent intramolecular complex. Analysis of the FCS data of the flavoprotein NADPH-cytochrome P450 oxidore
- In this work we show that the dyes coumarin 6, hypericin, 7-O-ethylresorufin and resorufin are suitable for fluorescence correlation spectroscopy (FCS) and demonstrate the use of these dyes in physiologically relevant protein studies. Since coumarins are metabolised by cytochromes P450, the binding of coumarin 6 to cytochrome P450 3A4 was investigated by FCS. Coumarin 6 appears to be a very bright non-covalent cytochrome P450 label. When titrating cytochrome P450 3A4 with coumarin 6, the diffusion time of the coumarin 6/ cytochrome P450 3A4 complex increases roughly two-fold at protein concentrations higher than 1 μmol l<sup>-1</sup>, indicating the formation of cytochrome aggregates. FCS of the flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) shows that both endogenous dyes undergo photobleaching. Moreover, FAD appears to be present to great extent, as a non-fluorescent intramolecular complex. Analysis of the FCS data of the flavoprotein NADPH-cytochrome P450 oxidore (en)
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| Title
| - Coumarin 6, hypericin, resorufins, and flavins: suitable chromophores for fluorescence correlation spectroscopy of biological molecules
- Coumarin 6, hypericin, resorufins, and flavins: suitable chromophores for fluorescence correlation spectroscopy of biological molecules (en)
- Kumarin 6, hypericin, resorufiny a flaviny: vhodné chromofory pro fluorescenční korelační spektroskopii biologických molekul (cs)
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| skos:prefLabel
| - Coumarin 6, hypericin, resorufins, and flavins: suitable chromophores for fluorescence correlation spectroscopy of biological molecules
- Coumarin 6, hypericin, resorufins, and flavins: suitable chromophores for fluorescence correlation spectroscopy of biological molecules (en)
- Kumarin 6, hypericin, resorufiny a flaviny: vhodné chromofory pro fluorescenční korelační spektroskopii biologických molekul (cs)
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| skos:notation
| - RIV/61989592:15110/01:00006962!RIV09-MSM-15110___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA203/99/0277), P(LN00A032), Z(AV0Z4040901), Z(MSM 151100003)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/01:00006962
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Fluorescence correlation spectroscopy; Cytochrome P450; NADPH-cytochrome P450 oxidoreductase; Single molecule spectroscopy (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Collection of Czechoslovak Chemical Communications
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hof, Martin
- Hudeček, Jiří
- Anzenbacher, Pavel
- Beneš, Martin
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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