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  • Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with alpha- or beta-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (similar to 58 mol %), mannose (similar to 22 mol %), and galactose (similar to 18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue alpha-D-Glcp-(1 -> 2)-beta-D-Galf-(1 -> 6)-alpha-D-Manp-(1 -> 3)-L-Ser. Terminal alpha-D-Glcp and (1 -> 6)-beta-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrated induce defence response in B. napus plants.
  • Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with alpha- or beta-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (similar to 58 mol %), mannose (similar to 22 mol %), and galactose (similar to 18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue alpha-D-Glcp-(1 -> 2)-beta-D-Galf-(1 -> 6)-alpha-D-Manp-(1 -> 3)-L-Ser. Terminal alpha-D-Glcp and (1 -> 6)-beta-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrated induce defence response in B. napus plants. (en)
Title
  • Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus
  • Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus (en)
skos:prefLabel
  • Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus
  • Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus (en)
skos:notation
  • RIV/61389030:_____/13:00397255!RIV14-MZE-61389030
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/08/1581), P(QH81201), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 22
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 64484
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/13:00397255
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • elicitor; oligosaccharide; Leptosphaeria maculans (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [42E0A2826B01]
http://linked.open...i/riv/nazevZdroje
  • Journal of Agricultural and Food Chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 61
http://linked.open...iv/tvurceVysledku
  • Burketová, Lenka
  • Valentová, O.
  • Synytsya, A.
  • Jindřichová, Bára
  • Čopíková, J.
  • Šašek, Vladimír
  • Kim, P. D.
http://linked.open...ain/vavai/riv/wos
  • 000320153500003
http://linked.open...n/vavai/riv/zamer
issn
  • 0021-8561
number of pages
http://bibframe.org/vocab/doi
  • 10.1021/jf401221v
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