About: Chromosome Analysis and Sorting Using Flow Cytometry

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure for preparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can be induced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications. Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure for preparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can be induced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications. (en)
Title	Chromosome Analysis and Sorting Using Flow Cytometry Chromosome Analysis and Sorting Using Flow Cytometry (en)
skos:prefLabel	Chromosome Analysis and Sorting Using Flow Cytometry Chromosome Analysis and Sorting Using Flow Cytometry (en)
skos:notation	RIV/61389030:_____/11:00375427!RIV12-AV0-61389030
http://linked.open...avai/predkladatel	Ústav experimentální botaniky AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/07/1573), P(LC06004), Z(AV0Z50380511)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Doležel, Jaroslav Šimková, Hana Kubaláková, Marie Suchánková, Pavla Číhalíková, Jarmila
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	190340
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/11:00375427
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chromosome analysis; flow cytometry; root meristems (en)
http://linked.open.../riv/klicoveSlovo	Chromosome analysis flow cytometry root meristems
http://linked.open...ontrolniKodProRIV	[B32634A98363]
http://linked.open...i/riv/mistoVydani	New Jersey
http://linked.open...i/riv/nazevZdroje	Plant Chromosome Engineering: Methods and Protocols
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...v/pocetStranKnihy	329 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Creation of resources for wheat genomics and map-based cloning of resistance genes from chromosome 7D Integration of research activities to study the plant genome
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Kubaláková, Marie Suchánková, Pavla Číhalíková, Jarmila Šimková, Hana
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
number of pages	18 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-61737-957-4_12
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-61737-956-7
is http://linked.open...avai/riv/vysledek of	Chromosome Analysis and Sorting Using Flow Cytometry Chromosome Analysis and Sorting Using Flow Cytometry

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