About: The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The non-photosynthetic NADP-malic enzyme EC 1.1.1.40 (NADP-ME), which catalyzes the oxidative decarboxylation of L-malate and NADP(+) to produce pyruvate and NADPH, respectively, and which could be involved in plant defense responses, was isolated from Nicotiana tabacum L. leaves. The mechanism of the enzyme reaction was studied by the initial rate method and was found to be an ordered sequential one. Regulation possibilities of purified cytosolic NADP-ME by cell metabolites were tested. Intermediates of the citric acid cycle (a-ketoglutarate, succinate, fumarate), metabolites of glycolysis (pyruvate, phosphoenolpyruvate, glucose-6-phosphate), compounds connected with lipogenesis (coenzyme A, acetyl-CoA, palmitoyl-CoA) and some amino acids (glutamate, glutamine, aspartate) did not significantly affect the NADP-ME activity from tobacco leaves. In contrast, macroergic compounds (GTP, ATP and ADP) were strong inhibitors of NADP-ME. The non-photosynthetic NADP-malic enzyme EC 1.1.1.40 (NADP-ME), which catalyzes the oxidative decarboxylation of L-malate and NADP(+) to produce pyruvate and NADPH, respectively, and which could be involved in plant defense responses, was isolated from Nicotiana tabacum L. leaves. The mechanism of the enzyme reaction was studied by the initial rate method and was found to be an ordered sequential one. Regulation possibilities of purified cytosolic NADP-ME by cell metabolites were tested. Intermediates of the citric acid cycle (a-ketoglutarate, succinate, fumarate), metabolites of glycolysis (pyruvate, phosphoenolpyruvate, glucose-6-phosphate), compounds connected with lipogenesis (coenzyme A, acetyl-CoA, palmitoyl-CoA) and some amino acids (glutamate, glutamine, aspartate) did not significantly affect the NADP-ME activity from tobacco leaves. In contrast, macroergic compounds (GTP, ATP and ADP) were strong inhibitors of NADP-ME. (en)
Title	The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves (en)
skos:prefLabel	The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves (en)
skos:notation	RIV/61389030:_____/09:00337488!RIV10-AV0-61389030
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M0505), S, Z(AV0Z50380511), Z(MSM0021620808)
http://linked.open...iv/cisloPeriodika	8-9
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Müller, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	338688
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/09:00337488
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	NADP-malic enzyme; macroergic compounds; Nicotiana tabacum L. (en)
http://linked.open.../riv/klicoveSlovo	NADP-malic enzyme Nicotiana tabacum L. macroergic compounds
http://linked.open...odStatuVydavatele	CS - Státní svaz Srbska a Černé Hory
http://linked.open...ontrolniKodProRIV	[4183B8C0C456]
http://linked.open...i/riv/nazevZdroje	Journal of the Serbian Chemical Society
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Müller, Karel Doubnerová, V. Ryšlavá, H. Potůčková, L.
http://linked.open...ain/vavai/riv/wos	000270267200005
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu
issn	0352-5139
number of pages	14 (xsd:int)
is http://linked.open...avai/riv/vysledek of	The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves

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