About: Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The complete genomes of three Czech isolates VIRUBRA 1/045, VIRUBRA 1/046, and VIRUBRA 1/047 of Potato leafroll virus (PLRV) were sequenced and compared with 13 complete sequences of PLRV isolates available in GenBank. Among the Czech isolates, VIRUBRA 1/046 and 1/047 showed the highest nucleotide (nt) identity (98.7%). PLRV was the most conserved virus in both open reading frames (ORFs) 3 and 4. The most variable regions were ORFs 0 and Rap1. Interestingly, isolate VIRUBRA 1/045 significantly differed from the other two Czech isolates in ORFs 0 and 1. Moreover, we identified mutations in the amino acid (aa) sequences, which were specific for the Czech isolates. Phylogenetic analysis based on ORF0 showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on sequence analysis of the genome sequences of PLRV isolates from the Czech Republic. The complete genomes of three Czech isolates VIRUBRA 1/045, VIRUBRA 1/046, and VIRUBRA 1/047 of Potato leafroll virus (PLRV) were sequenced and compared with 13 complete sequences of PLRV isolates available in GenBank. Among the Czech isolates, VIRUBRA 1/046 and 1/047 showed the highest nucleotide (nt) identity (98.7%). PLRV was the most conserved virus in both open reading frames (ORFs) 3 and 4. The most variable regions were ORFs 0 and Rap1. Interestingly, isolate VIRUBRA 1/045 significantly differed from the other two Czech isolates in ORFs 0 and 1. Moreover, we identified mutations in the amino acid (aa) sequences, which were specific for the Czech isolates. Phylogenetic analysis based on ORF0 showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on sequence analysis of the genome sequences of PLRV isolates from the Czech Republic. (en)
Title	Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic (en)
skos:prefLabel	Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic (en)
skos:notation	RIV/61389030:_____/09:00328620!RIV10-MZE-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06030), P(QH71123), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Čeřovská, Noemi Moravec, Tomáš Plchová, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	341073
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/09:00328620
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Potato leafroll virus; Sequencing; Phylogeny (en)
http://linked.open.../riv/klicoveSlovo	Potato leafroll virus Phylogeny Sequencing
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[9DCB69FBE4FC]
http://linked.open...i/riv/nazevZdroje	Virus Genes
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Variability of Potato leafroll luteovirus (PLRV), improved reliability of its detection and utilization of transgenic resistance. Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	39
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Dědič, P. Plchová, Helena Čeřovská, Noemi
http://linked.open...ain/vavai/riv/wos	000267299900021
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0920-8569
number of pages	3 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic

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