About: Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells.

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About: Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The growth and viability of tobacco cells (Nicotiana tabacum L.) immobilized in alginate or pectate were' monitored during their cultivation by using two-dimensional fluorescence spectroscopy (2-D FS). The cell growth was followed via the fluorescence of amino acids in proteins. The correlation between the tryptophan fluorescence and the cell biomass inside the alginate beads was verified by comparison with the dry weight of the cells. The determination of biomass content or cell viability by measurement of the intensity of NAD(P)H fluorescence was found unsuitable. Cell viability was estimated by determination of cell esterase activity using fluorescein diacetate as a fluorogenic substrate. The fluorescence intensities of both fluorophores, tryptophan and fluorescein, were determined by scanning a 2-D FS spectrum of intact beads in front face cuvette. Using this technique the effect of organic solvent, dimethyl sulfoxide, on the growth and metabolic activities of cells within the beads was evaluated. The growth and viability of tobacco cells (Nicotiana tabacum L.) immobilized in alginate or pectate were' monitored during their cultivation by using two-dimensional fluorescence spectroscopy (2-D FS). The cell growth was followed via the fluorescence of amino acids in proteins. The correlation between the tryptophan fluorescence and the cell biomass inside the alginate beads was verified by comparison with the dry weight of the cells. The determination of biomass content or cell viability by measurement of the intensity of NAD(P)H fluorescence was found unsuitable. Cell viability was estimated by determination of cell esterase activity using fluorescein diacetate as a fluorogenic substrate. The fluorescence intensities of both fluorophores, tryptophan and fluorescein, were determined by scanning a 2-D FS spectrum of intact beads in front face cuvette. Using this technique the effect of organic solvent, dimethyl sulfoxide, on the growth and metabolic activities of cells within the beads was evaluated. (en)
Title	Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells. Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells. (en)
skos:prefLabel	Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells. Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells. (en)
skos:notation	RIV/61389030:_____/03:56033044!RIV/2004/AV0/A56004/N
http://linked.open.../vavai/riv/strany	632;635
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(OC 840.10), P(OC 843.10), Z(AV0Z4055905), Z(AV0Z4072921), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Vaňková, Radomíra Gaudinová, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	632349
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033044
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Two-Dimensional Fluorescence Spectroscopy; Immobilized Plant Cells; Tobacco (en)
http://linked.open.../riv/klicoveSlovo	Tobacco Immobilized Plant Cells Two-Dimensional Fluorescence Spectroscopy
http://linked.open...odStatuVydavatele	HR - Chorvatská republika
http://linked.open...ontrolniKodProRIV	[3A7AFC9EE611]
http://linked.open...i/riv/nazevZdroje	Chemical industry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20843.10 http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20840.10
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Kuncová, Gabriela Podrazký, Ondřej Vaněk, Tomáš Vaňková, Radomíra Gaudinová, Alena
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function Theoretical fundamentals of chemical processes: equilibrium and dynamic behavior of multiphase reacting systems Chemical principles of selected biological phenomena in medicine and ecology
issn	0354-7531
number of pages	4 (xsd:int)

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