About: solation, characterization and chromosome localization of repetitive DNA sequences in bananas (Musa spp.).

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About: solation, characterization and chromosome localization of repetitive DNA sequences in bananas (Musa spp.). Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Partial genomic DNA libraries were constructed in Musa acuminata and M. balbisiana and screened for clones carrying repeated sequences, and sequences carrying rDNA. Isolated clones were characterized in terms of copy number, genomic distribution in M. acuminata and M. balbisiana, and sequence similarity to known DNA sequences. Ribosomal RNA genes have been the most abundant sequences recovered. FISH with probes for DNA clones Radkal and Radkal, which carry different fragments of Musa 26S rDNA, and Radkal^, for which no homology with known DNA sequences has been found, resulted in clear signals at secondary constrictions. Only one clone carrying 5S rDNA, named Radkal, has been recovered. All remaining DNA clones exhibited more or less pronounced clustering at centromeric regions. The study revealed small differences in genomic distribution of repetitive DNA sequences between M. acuminata and M. balbisiana, the only exception being the 5S rDNA where the two Musa clones under study differed in the number Partial genomic DNA libraries were constructed in Musa acuminata and M. balbisiana and screened for clones carrying repeated sequences, and sequences carrying rDNA. Isolated clones were characterized in terms of copy number, genomic distribution in M. acuminata and M. balbisiana, and sequence similarity to known DNA sequences. Ribosomal RNA genes have been the most abundant sequences recovered. FISH with probes for DNA clones Radkal and Radkal, which carry different fragments of Musa 26S rDNA, and Radkal^, for which no homology with known DNA sequences has been found, resulted in clear signals at secondary constrictions. Only one clone carrying 5S rDNA, named Radkal, has been recovered. All remaining DNA clones exhibited more or less pronounced clustering at centromeric regions. The study revealed small differences in genomic distribution of repetitive DNA sequences between M. acuminata and M. balbisiana, the only exception being the 5S rDNA where the two Musa clones under study differed in the number (en)
Title	solation, characterization and chromosome localization of repetitive DNA sequences in bananas (Musa spp.). solation, characterization and chromosome localization of repetitive DNA sequences in bananas (Musa spp.). (en)
skos:prefLabel	solation, characterization and chromosome localization of repetitive DNA sequences in bananas (Musa spp.). solation, characterization and chromosome localization of repetitive DNA sequences in bananas (Musa spp.). (en)
skos:notation	RIV/61389030:_____/02:56023016!RIV/2003/AV0/A56003/N
http://linked.open.../vavai/riv/strany	89;100
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA6038204), P(IBS5038104), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Šafář, Jan Valárik, Miroslav Doležel, Jaroslav Šimková, Hana Kubaláková, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	664122
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/02:56023016
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chromosome structure; genome size; in situ hybridization (en)
http://linked.open.../riv/klicoveSlovo	genome size in situ hybridization chromosome structure
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[31A87F8F9BA1]
http://linked.open...i/riv/nazevZdroje	Chromosome research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Construction of banana bacterial artificial chromosome (BAC) library for physical genome mapping Molecular cytogenetic and cytometric methods for breeding of grasses and trifolium
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Valárik, Miroslav Šafář, Jan Šimková, Hana Doleželová, Marie
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0967-3849
number of pages	12 (xsd:int)

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