About: Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies

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About: Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Towards potential treatment of neurodegenerative disorders by nanomedicine gene therapy, a nonviral gene delivery system is created by complexation of novel PEGylated cationic lipid assemblies and a plasmid DNA encoding for the protein brain-derived neurotrophic factor (BDNF). The generated monoolein-based liquid crystalline nanocarriers for BDNF gene transfer are characterized by means of small-angle X-ray scattering (SAXS), cryogenic transmission electron microscopy (cryo-TEM) techniques, and turbidity measurements. Morphologically variable populations of sterically stabilized, PEGylated multicompartment nanoobjects, with confined supercoiled DNA, are established by cryo-TEM imaging. Synchrotron radiation SAXS measurements, performed with diluted nanoparticulate dispersions, evidence the inner multilamelar structure of pear-type vesicles and flexible onion-like objects functionalized by pBDNF. Obtained structural data are of fundamental interest for improvement of brain-targeted gene delivery. Towards potential treatment of neurodegenerative disorders by nanomedicine gene therapy, a nonviral gene delivery system is created by complexation of novel PEGylated cationic lipid assemblies and a plasmid DNA encoding for the protein brain-derived neurotrophic factor (BDNF). The generated monoolein-based liquid crystalline nanocarriers for BDNF gene transfer are characterized by means of small-angle X-ray scattering (SAXS), cryogenic transmission electron microscopy (cryo-TEM) techniques, and turbidity measurements. Morphologically variable populations of sterically stabilized, PEGylated multicompartment nanoobjects, with confined supercoiled DNA, are established by cryo-TEM imaging. Synchrotron radiation SAXS measurements, performed with diluted nanoparticulate dispersions, evidence the inner multilamelar structure of pear-type vesicles and flexible onion-like objects functionalized by pBDNF. Obtained structural data are of fundamental interest for improvement of brain-targeted gene delivery. (en)
Title	Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies (en)
skos:prefLabel	Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies (en)
skos:notation	RIV/61389013:_____/11:00364996!RIV12-AV0-61389013
http://linked.open...avai/predkladatel	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/09/2078), P(GAP208/10/1600), Z(AV0Z40500505)
http://linked.open...iv/cisloPeriodika	20
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Štěpánek, Petr Filippov, Sergey Angelov, Borislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	235551
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/11:00364996
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA-lipid-surfactant complexes; CryoTEM; small-angle X-ray scattering (en)
http://linked.open.../riv/klicoveSlovo	CryoTEM DNA-lipid-surfactant complexes small-angle X-ray scattering
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[AC0FC4DE1F92]
http://linked.open...i/riv/nazevZdroje	Soft Matter
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Polymeric particles and nanostructured materials stabilized by surface active molecules Bioinspired nanoparticles sensitive to environment stimuli
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Filippov, Sergey Štěpánek, Petr Karlsson, G. Angelov, Borislav Angelova, A. Lesieur, S. Terrill, N.
http://linked.open...ain/vavai/riv/wos	000295582000022
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
issn	1744-683X
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1039/C1SM06447A
is http://linked.open...avai/riv/vysledek of	Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies

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