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Description
| - Byly získány nové poznatky o struktuře tmRNA průmyslově významných producentů antibiotik S. aureofaciens, S. griseus a S. coelicolor. 2) Byla objasněna asociaci proteinů s tmRNA v průběhu růstu S. aureofaciens. 3) Byla vysvětlena funkce ribosomálního proteinu SS1 při trans-translaci. 4) Získali jsme původní informace o struktuře proteinu SmpB u S. aureofaciens a jeho hladinách u buněk z exponenciální a stacionární fáze růstu (cs)
- Comparative sequence analysis of ssrA genes of streptomycetes revealed the most significant differences in the central parts of tag-reading frames, in the stop codons and in the 15-34 nucleotide sequences following stop codons. Proteins from various phases of development of Streptomyces aureofaciens associated with tmRNA were analyzed. Using affinity chromatography on tmRNA-Sepharose and photo cross-linking experiments with [32P]labelled tmRNA seven proteins, were identified among the proteins associated with tmRNA of Streptomyces aureofaciens. We examined functional role of ribosomal protein SS1 in defined in vitro trans-translation system. Our data show that the protein SS1 that structurally differ from S1 of Escherichia coli is required for translation of the tmRNA tag-reading frame
- Comparative sequence analysis of ssrA genes of streptomycetes revealed the most significant differences in the central parts of tag-reading frames, in the stop codons and in the 15-34 nucleotide sequences following stop codons. Proteins from various phases of development of Streptomyces aureofaciens associated with tmRNA were analyzed. Using affinity chromatography on tmRNA-Sepharose and photo cross-linking experiments with [32P]labelled tmRNA seven proteins, were identified among the proteins associated with tmRNA of Streptomyces aureofaciens. We examined functional role of ribosomal protein SS1 in defined in vitro trans-translation system. Our data show that the protein SS1 that structurally differ from S1 of Escherichia coli is required for translation of the tmRNA tag-reading frame (en)
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Title
| - SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation
- SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation (en)
- SsrA geny streptomycet a asociace proteinů s tmRNA během vývoje a buněčné diferenciace (cs)
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skos:prefLabel
| - SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation
- SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation (en)
- SsrA geny streptomycet a asociace proteinů s tmRNA během vývoje a buněčné diferenciace (cs)
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skos:notation
| - RIV/61388971:_____/08:00313096!RIV09-AV0-61388971
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA203/05/0106), S, Z(AV0Z50200510)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/08:00313096
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - in vitro trans-translation; ribosomal protein ss1; streptomycetes (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Halada, Petr
- Felsberg, Jürgen
- Bobek, Jan
- Mikulík, Karel
- Zídková, J.
- Palečková, Petra
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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