About: eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA     Goto   Sponge   NotDistinct   Permalink

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  • Yeast initiation factor eIF3 (eukaryotic initiation factor 3) has been implicated in multiple steps of translation initiation. Previously, we showed that the N-terminal domain (NTD) of eIF3a interacts with the small ribosomal protein RPS0A located near the mRNA exit channel, where eIF3 is proposed to reside. Here, we demonstrate that a partial deletion of the RPS0A-binding domain of eIF3a impairs translation initiation and reduces binding of eIF3 and associated eIFs to native preinitiation complexes in vivo. Strikingly, it also severely blocks the induction of GCN4 translation that occurs via reinitiation. Detailed examination unveiled a novel reinitiation defect resulting from an inability of 40S ribosomes to resume scanning after terminating at the first upstream ORF (uORF1). Genetic analysis reveals a functional interaction between the eIF3a-NTD and sequences 5_ of uORF1 that is critically required to enhance reinitiation
  • Yeast initiation factor eIF3 (eukaryotic initiation factor 3) has been implicated in multiple steps of translation initiation. Previously, we showed that the N-terminal domain (NTD) of eIF3a interacts with the small ribosomal protein RPS0A located near the mRNA exit channel, where eIF3 is proposed to reside. Here, we demonstrate that a partial deletion of the RPS0A-binding domain of eIF3a impairs translation initiation and reduces binding of eIF3 and associated eIFs to native preinitiation complexes in vivo. Strikingly, it also severely blocks the induction of GCN4 translation that occurs via reinitiation. Detailed examination unveiled a novel reinitiation defect resulting from an inability of 40S ribosomes to resume scanning after terminating at the first upstream ORF (uORF1). Genetic analysis reveals a functional interaction between the eIF3a-NTD and sequences 5_ of uORF1 that is critically required to enhance reinitiation (en)
  • Práce pojednává o studiu iniciace translace a specifické úloze translačního iniciačního faktoru eIF3 (cs)
Title
  • eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA
  • eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA (en)
  • eIF3 hraje kritickou úlohu v procesu reiniciace, který řídí expresi kvasinkového genu GCN4, prostřednictvým interakce s 5’ oblastí uORF1 nacházející se v netranslatované oblasti GCN4 (cs)
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  • eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA
  • eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA (en)
  • eIF3 hraje kritickou úlohu v procesu reiniciace, který řídí expresi kvasinkového genu GCN4, prostřednictvým interakce s 5’ oblastí uORF1 nacházející se v netranslatované oblasti GCN4 (cs)
skos:notation
  • RIV/61388971:_____/08:00312545!RIV09-AV0-61388971
http://linked.open...avai/riv/aktivita
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  • Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika
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http://linked.open...aciTvurceVysledku
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http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 365522
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/08:00312545
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  • translation initiation; reinitiation; eif3 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [15E84E57B131]
http://linked.open...i/riv/nazevZdroje
  • Genes and Development
http://linked.open...in/vavai/riv/obor
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http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 22
http://linked.open...iv/tvurceVysledku
  • Valášek, Leoš
  • Munzarová, Vanda
  • Szamecz, Bela
  • Rutkai, Edit
  • Herrmannová, Anna
  • Cuchalová, Lucie
  • Hinnebusch, A. G.
  • Burela, L.
  • Nielsen, K. H.
http://linked.open...ain/vavai/riv/wos
  • 000258878100012
http://linked.open...n/vavai/riv/zamer
issn
  • 0890-9369
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