About: Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes

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About: Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Práce dokazuje, že proces dekolorizace syntetických barviv Reactive Orange 16 a Remazol Brilliant Blue R houbou Irpex lacteus je spojen oxidativním enzymem podobným lakasam a v tomto případě asociovaným s houbovým myceliem. Ve své vázané formě enzym vykazoval optimum pro oxidace ABTS, DMP a guaiacolu při pH 3.0 a při pH 7,0 pro oxidaci syringaldazinu. Nejvyšší enzymová aktivita byla při tom naměřena s ABTS. Aktivita byla inhibována 50 mM NaN3. Přidání redox mediátorů mělo pozitivní efekt na dekolorizaci barviv pomocí houbového mycelia. Enzym uvolněný z mycelia byl také schopen odbarvovat vybraná barviva za in vitro podmínek (cs) The white rot fungus Irpex lacteus is able to decolorize such synthetic dyes as Reactive Orange 16 and Remazol Brilliant Blue R. Here,we demonstrate that this type of dye decolorization is mainly related to a laccase-like enzyme activity associated with fungal mycelium. In its bound form, the enzyme detected showed a pH optimum of 3.0 for the oxidation of ABTS, DMP and guaiacol, and a pH of 7.0 for syringaldazine. The highest enzymatic activity was obtained with ABTS as substrate. Enzyme activity was fully inhibited with 50 mM NaN3. Depending on the chemical structure of dyes, redox mediators had a positive effect on the dye decolorization by fungal mycelium.Enzyme isolated from fungal mycelium was able to decolorize synthetic dyes in vitro The white rot fungus Irpex lacteus is able to decolorize such synthetic dyes as Reactive Orange 16 and Remazol Brilliant Blue R. Here,we demonstrate that this type of dye decolorization is mainly related to a laccase-like enzyme activity associated with fungal mycelium. In its bound form, the enzyme detected showed a pH optimum of 3.0 for the oxidation of ABTS, DMP and guaiacol, and a pH of 7.0 for syringaldazine. The highest enzymatic activity was obtained with ABTS as substrate. Enzyme activity was fully inhibited with 50 mM NaN3. Depending on the chemical structure of dyes, redox mediators had a positive effect on the dye decolorization by fungal mycelium.Enzyme isolated from fungal mycelium was able to decolorize synthetic dyes in vitro (en)
Title	Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes (en) Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes (cs)
skos:prefLabel	Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes (en) Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes (cs)
skos:notation	RIV/61388971:_____/07:00097671!RIV08-AV0-61388971
http://linked.open.../vavai/riv/strany	463;471
http://linked.open...avai/riv/aktivita	O P R Z
http://linked.open...avai/riv/aktivity	O, P(IAA6020411), R, Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Svobodová, Kateřina Novotný, Čeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	425714
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/07:00097671
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	irpex lacteus; dye decolorization; laccase (en)
http://linked.open.../riv/klicoveSlovo	dye decolorization laccase irpex lacteus
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[4E4090195E96]
http://linked.open...i/riv/nazevZdroje	Bioresource Technology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Mechanisms of biodegradation of environemntal organopollutants by ligninolytic fungi
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	99
http://linked.open...iv/tvurceVysledku	Svobodová, Kateřina Novotný, Čeněk Kuees, U. Majcherczyk, A.
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0960-8524
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes

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