About: Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis     Goto   Sponge   NotDistinct   Permalink

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  • Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract–(NH4)2SO4–glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium
  • Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract–(NH4)2SO4–glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium (en)
  • Streptomyces caelestis DSM 40 084 produkuje dva osmolyty: 2-O-(D-glukopyranosyl-glycerolovou kyselinu (GG) a trehalosu. Obě sloučeniny byly izolovány a identifikovány nukleární magnetickou resonancí a hmotnostní spektrometrií. Byla zjištěna velmi citlivá regulace buněčných osmolytů v exponenciálně rostoucích kulturách. Intracelulární obsah GG a trehalosy se zvýšil dvakrát po skokové změně osmolarity média vyvolané 0.3 procent NaCl. 1H NMR analýza buněčných extraktů nepotvrdila přítomnost dalších osmolytů. GG je metabolit uvolňovaný z buněk a jeho koncentrace dosahuje 3g/L při kultivaci v médiu obsahující yeast extrakt, (NH4)2SO4 a glycerol. Tato práce poprvé informuje o výskytu iontového osmolytu GG u rodu Streptomyces a o jeho volné exkreci do média (cs)
Title
  • Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis
  • Glukosylglycerát je osmolit a extracelulární metabolit produkovaný Streptomyces caelestis (cs)
  • Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis (en)
skos:prefLabel
  • Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis
  • Glukosylglycerát je osmolit a extracelulární metabolit produkovaný Streptomyces caelestis (cs)
  • Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis (en)
skos:notation
  • RIV/61388971:_____/07:00096764!RIV08-AV0-61388971
http://linked.open.../vavai/riv/strany
  • 451;456
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAA600660607), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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  • 423486
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/07:00096764
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • streptomyces caelestis; mass spectrometry (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [CE726D1D94D9]
http://linked.open...i/riv/nazevZdroje
  • Folia Microbiologica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 52
http://linked.open...iv/tvurceVysledku
  • Halada, Petr
  • Pospíšil, Stanislav
  • Petříček, Miroslav
  • Sedmera, Petr
http://linked.open...n/vavai/riv/zamer
issn
  • 0015-5632
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