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| Description
| - We have investigated the pathway by which the 16 amino-acid C-terminal extension of the D1 subunit of photosystem two is removed in the cyanobacterium Synechocystis sp. PCC 6803 to leave Ala344 as the C-terminal residue. Previous work has suggested a two-step process involving formation of a processing intermediate of D1, termed iD1, of uncertain origin. Here we show by mass spectrometry that a synthetic peptide mimicking the C- terminus of the D1 precursor is cleaved by cellular extracts or purified CtpA processing protease after residue Ala352, making this a likely site for formation of iD1. Characteristics of D1 site-directed mutants with either the Leu353 residue replaced by Pro or with a truncation after Ala352 are in agreement with this assignment
- We have investigated the pathway by which the 16 amino-acid C-terminal extension of the D1 subunit of photosystem two is removed in the cyanobacterium Synechocystis sp. PCC 6803 to leave Ala344 as the C-terminal residue. Previous work has suggested a two-step process involving formation of a processing intermediate of D1, termed iD1, of uncertain origin. Here we show by mass spectrometry that a synthetic peptide mimicking the C- terminus of the D1 precursor is cleaved by cellular extracts or purified CtpA processing protease after residue Ala352, making this a likely site for formation of iD1. Characteristics of D1 site-directed mutants with either the Leu353 residue replaced by Pro or with a truncation after Ala352 are in agreement with this assignment (en)
- Studovali jsme cestu, při které je C-koncová sekvence proteinu D1 v sinici Synechocystis sp. PCC 6803 odštěpena za vzniku maturovaného proteinu s koncovým zbytkem Ala344. Předcházející práce ukázaly, že se jedná o dvoukrokový process, při kterém vzniká intermediární forma proteinu iD1. S pomocí hmotnostní spektrometrie jsme ukázali, že syntetický peptid napodobující C- konec prekursoru D1 je štěpen buněčným extraktem nebo vyčištěnou maturační proteázou CtpA za zbytkem Ala352. Charakteristiky mutantů v proteinu D1 se zbytkem Leu353 vyměněným za Pro nebo s odtraněnou sekvencí za zbytkem Ala352 byůy v souladu s identifikovaným místem štěpení (cs)
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| Title
| - Cleavage after residue Ala352 in the C-terminal extension is an early step in the maturation of the D1 subunit of Photosystem II in Synechocystis PCC 6803
- Štěpení za zbytkem Ala352 je časným krokem během maturace podjednotky D1 komplexu fotosystému II u sinice Synechocystis PCC 6803 (cs)
- Cleavage after residue Ala352 in the C-terminal extension is an early step in the maturation of the D1 subunit of Photosystem II in Synechocystis PCC 6803 (en)
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| skos:prefLabel
| - Cleavage after residue Ala352 in the C-terminal extension is an early step in the maturation of the D1 subunit of Photosystem II in Synechocystis PCC 6803
- Štěpení za zbytkem Ala352 je časným krokem během maturace podjednotky D1 komplexu fotosystému II u sinice Synechocystis PCC 6803 (cs)
- Cleavage after residue Ala352 in the C-terminal extension is an early step in the maturation of the D1 subunit of Photosystem II in Synechocystis PCC 6803 (en)
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| skos:notation
| - RIV/61388971:_____/07:00090147!RIV08-AV0-61388971
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - Z(AV0Z50200510), Z(MSM6007665808)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/07:00090147
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - ctpa protease; d1 maturation; photosystem II (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Biochimica Et Biophysica Acta-Bioenergetics
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Komenda, Josef
- Eichacker, L. A.
- Nixon, P. J.
- Kuviková, Stanislava
- Diner, B. A.
- Granvogl, B.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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