About: Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We constructed a reporter gene system, based on luciferase genes from Vibrio harvei, for measurement of translation nonsense suppression in Streptomyces. Streptomyces lividans TK 24 was transformed with the plasmids and the reporter system was tested by growth of the strain in the presence of streptomycin as a translation accuracy modulator. Streptomycin increased nonsense suppression on UAA nearly 10-fold and more than 20-fold on UAG. Whereas on UGA, the most frequent stop signal in Streptomyces, the effect was negligible We constructed a reporter gene system, based on luciferase genes from Vibrio harvei, for measurement of translation nonsense suppression in Streptomyces. Streptomyces lividans TK 24 was transformed with the plasmids and the reporter system was tested by growth of the strain in the presence of streptomycin as a translation accuracy modulator. Streptomycin increased nonsense suppression on UAA nearly 10-fold and more than 20-fold on UAG. Whereas on UGA, the most frequent stop signal in Streptomyces, the effect was negligible (en) Sestrojili jsme genový reporterový systém využívající luciferázu z Vibrio harvei pro měření translační nonsense suprese u streptomycet. Transformovali jsme Streptomyces lividans TK 24 plasmidem nesoucím reporterový systém, který jsme testovali během růstu kultury v přítomnosti a nepřítomnosti streptomycinu jako modulátoru přesnosti translace. Streptomycin zvyšoval nonsense supresi UAA téměř desetkrát a v případě UAG více než dvacetkrát. V případě UGA, nejčastějšího stop signálu u streptomycet, byl efekt zanedbatelný (cs)
Title	Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces Konstrukce a testování reporterového systému, založeného na bakteriální luciferáze, určeného pro měření nonsense suprese u streptomycet (cs) Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces (en)
skos:prefLabel	Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces Konstrukce a testování reporterového systému, založeného na bakteriální luciferáze, určeného pro měření nonsense suprese u streptomycet (cs) Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces (en)
skos:notation	RIV/61388971:_____/06:00039435!RIV07-GA0-61388971
http://linked.open.../vavai/riv/strany	62;64
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/03/0292), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Buriánková, Karolína Kalachová, Ladislava Branny, Pavel Weiser, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	469654
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/06:00039435
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	streptomyces; reporter gene system (en)
http://linked.open.../riv/klicoveSlovo	streptomyces reporter gene system
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[4645302A0BC2]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Proteomic analysis of adaptive mutations in antibiotic resistant bacteria during long time cultivations
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Branny, Pavel Pernodet, J.-L. Weiser, Jaroslav Buriánková, Karolína Kalachová, Ladislava
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0015-5632
number of pages	3 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces

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