About: Effect of Phosphate on the Expression of Protein-Ser/Thr Kinase Pkg2 in Streptomyces granaticolor

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A time-correlated expression of eukaryotic-like Ser/Thr protein kinase Pkg2 of Streptomyces granaticolor was investigated by RT-PCR and by transcription fusion experiments. In a complex medium the activity of pkg2 promotor was constant during the life cycle. Direct RNA analysis proved the presence of corresponding pkg2 transcript. S1 nuclease protection analysis of the transcription initiation site showed that pkg2 gene is a leaderless mRNA. Under phosphate starvation the promotor activity was detectable merely in the early exponential phase. Under these conditions turning off of the pkg2 promotor and cessation of pkg2 transcript level coincided with the start of granaticin production A time-correlated expression of eukaryotic-like Ser/Thr protein kinase Pkg2 of Streptomyces granaticolor was investigated by RT-PCR and by transcription fusion experiments. In a complex medium the activity of pkg2 promotor was constant during the life cycle. Direct RNA analysis proved the presence of corresponding pkg2 transcript. S1 nuclease protection analysis of the transcription initiation site showed that pkg2 gene is a leaderless mRNA. Under phosphate starvation the promotor activity was detectable merely in the early exponential phase. Under these conditions turning off of the pkg2 promotor and cessation of pkg2 transcript level coincided with the start of granaticin production (en) Exprese Pkg2, Ser/Thr proteinkinasy eukaryotního typu, u Streptomyces granaticolor byla studována pomocí RT-PCR a transkripční fúze s reportérovým genem. V komplexním médiu byla aktivita pkg2 promotoru konstantní v průběhu celé růstové křivky a přímá analýza RNA prokázala přítomnost transkriptu pkg2. Analýza transkripčního počátku pomocí S1 nukleázového mapování prokázala, že pkg2 mRNA postrádá vedoucí sekvenci. Během hladovění na fosfát byla promotorová aktivita pkg2 detekovatelná pouze v průběhu časné exponenciální fáze. Snížení aktivity pkg2 promotoru a úbytek transkriptu se časově překrývalo se začátkem produkce granaticinu (cs)
Title	Effect of Phosphate on the Expression of Protein-Ser/Thr Kinase Pkg2 in Streptomyces granaticolor Effect of Phosphate on the Expression of Protein-Ser/Thr Kinase Pkg2 in Streptomyces granaticolor (en) Vliv fosfátu na expresi Ser/Thr proteinkinasy Pkg2 u Streptomyces granaticolor (cs)
skos:prefLabel	Effect of Phosphate on the Expression of Protein-Ser/Thr Kinase Pkg2 in Streptomyces granaticolor Effect of Phosphate on the Expression of Protein-Ser/Thr Kinase Pkg2 in Streptomyces granaticolor (en) Vliv fosfátu na expresi Ser/Thr proteinkinasy Pkg2 u Streptomyces granaticolor (cs)
skos:notation	RIV/61388971:_____/05:00039375!RIV07-GA0-61388971
http://linked.open.../vavai/riv/strany	503;508
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/02/1423), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Janeček, Jiří Vomastek, Tomáš Nádvorník, Richard Branny, Pavel Nováková, Linda
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	519387
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/05:00039375
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	streptomyces granaticolor; rna analysis; pkg2 promotor (en)
http://linked.open.../riv/klicoveSlovo	pkg2 promotor rna analysis streptomyces granaticolor
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[496300C51E88]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Relationship between activity of protein Ser/Thr kinases and inter- and intracellular signalling in differentiating and pathogenical bacterial species
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Branny, Pavel Janeček, Jiří Nováková, Linda Vomastek, Tomáš Nádvorník, Richard
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0015-5632
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Effect of Phosphate on the Expression of Protein-Ser/Thr Kinase Pkg2 in Streptomyces granaticolor

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