| Attributes | Values |
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| rdf:type
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| Description
| - Chemoenzymatická příprava 2-deoxy-2-propionamido-D-mannosy, 2-butyramido-2-deoxy-D-mannosy a 2-deoxy-2-phenylacetamido-D-mannosy zahrnovala N-acylaci 2-amino-2-deoxy-D-glukosy, její alkalickou epimerizaci a následnou selektivní mikrobiální degradaci gluko-epimerů. Poslední krok byl proveden pomocí buněk Rhodococcus equi A4 schopných degradovat 2-deoxy-2-propionamido-D-glukosu, 2-butyramido-2-deoxy-D-glukosu a 2-deoxy-2-phenylacetamido-D-glukosu, ale ne příslušné manno-isomery. 2-Acetamido-2-deoxy-6-O-phospho-D-glukosa amidohydrolasa [EC 3.5.1.25], ale ne 2-acetamido-2-deoxy-D-glukosa amidohydrolasa, byla detegována v buněčném extraktu. Enzym byl částečně purifikován (15.8krát s výtěžkem 18.1 % a specifickou aktivitou 0.95 U mg-1 proteinu) (cs)
- The chemoenzymatic route to 2-deoxy-2-propionamido-D-mannose, 2-butyramido-2-deoxy-D-mannose and 2-deoxy-2-phenylacetamido-D-mannose involved N-acylation of 2-amino-2-deoxy-D-glucose followed by alkaline C-2 epimerization and selective microbial removal of the epimers with gluco-configuration. The latter step employed whole cells of Rhodococcus equi A4 able to degrade 2-deoxy-2-propionamido-D-glucose, 2-butyramido-2-deoxy-D-glucose and 2-deoxy-2-phenylacetamido-D-glucose but inactive towards the corresponding manno-isomers. 2-Acetamido-2-deoxy-6-O-phospho-D-glucose amidohydrolase [EC 3.5.1.25] but not 2-acetamido-2-deoxy-D-glucose amidohydrolase was detected in the cell extract, the former enzyme being partially purified (15.8-fold with an overall yield of 18.1 % and a specific activity of 0.95 units mg-1 protein)
- The chemoenzymatic route to 2-deoxy-2-propionamido-D-mannose, 2-butyramido-2-deoxy-D-mannose and 2-deoxy-2-phenylacetamido-D-mannose involved N-acylation of 2-amino-2-deoxy-D-glucose followed by alkaline C-2 epimerization and selective microbial removal of the epimers with gluco-configuration. The latter step employed whole cells of Rhodococcus equi A4 able to degrade 2-deoxy-2-propionamido-D-glucose, 2-butyramido-2-deoxy-D-glucose and 2-deoxy-2-phenylacetamido-D-glucose but inactive towards the corresponding manno-isomers. 2-Acetamido-2-deoxy-6-O-phospho-D-glucose amidohydrolase [EC 3.5.1.25] but not 2-acetamido-2-deoxy-D-glucose amidohydrolase was detected in the cell extract, the former enzyme being partially purified (15.8-fold with an overall yield of 18.1 % and a specific activity of 0.95 units mg-1 protein) (en)
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| Title
| - A chemoenzymatic route to mannosamine derivatives bearing different N-acyl groups
- Chemoenzymatická příprava derivátů mannosaminů nesoucích různé N-acylové skupiny (cs)
- A chemoenzymatic route to mannosamine derivatives bearing different N-acyl groups (en)
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| skos:prefLabel
| - A chemoenzymatic route to mannosamine derivatives bearing different N-acyl groups
- Chemoenzymatická příprava derivátů mannosaminů nesoucích různé N-acylové skupiny (cs)
- A chemoenzymatic route to mannosamine derivatives bearing different N-acyl groups (en)
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| skos:notation
| - RIV/61388971:_____/05:00021859!RIV06-AV0-61388971
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA524/00/1275), P(OC D25.001), Z(AV0Z50200510)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/05:00021859
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - rhodococcus equi; N-Acylated 2-amino-2-deoxy-D-glucosamines (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bezouška, Karel
- Křen, Vladimír
- Kuzma, Marek
- Martínková, Ludmila
- Pompach, Petr
- Kristová, Veronika
- Hušáková, Lucie
- Rauvolfová, Jana
- Kavan, D.
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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