About: Searching for a nuclear transportin of the chromatin-remodeling factor ISW1 in Saccharomyces cerevisiae

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About: Searching for a nuclear transportin of the chromatin-remodeling factor ISW1 in Saccharomyces cerevisiae Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bylo připraveno několik intragenově značených verzí Isw1, jejichž distribuce byla studována na živých či fixovaných buňkách. Kompletní sada importinových mutantů byla těmito konstrukty transformována. V delečních mutantech importinů Kap120p, Nmd5p, Sxm1p, Kap114p, Los1p, Msn5p, Kap123p a Pdr6 jsme zjistili, že Isw1-GFP byl vždy správně lokalizován do buněčného jádra, což ukazuje, že tyto importiny nejsou do transporu zapojeny. (cs) Several intragenously-tagged versions of the ISW1 gene were constructed to follow Isw1p localization either in living (GFP-fusions) or in fixed cells (c-Myc-fusions). The whole set of available importin mutants was transformed with vectors carrying an intragenously-tagged ISW1 gene. In the strains where the importins Kap120p, Nmd5p, Sxm1p, Kap114p, Los1p, Msn5p, Kap123p and Pdr6 were deleted, the Isw1p-GFP fusion regulated by the ISW1 promoter was localized always in the nucleus. This suggests that none from these importins is required for nuclear localization of Isw1p. The localization of the Isw1p-GFP in the mtr10-1 strain differed from that observed in all the other analyzed importin mutants. Our results indicate that Mtr10p could be a good candidate for Isw1p importin. Several intragenously-tagged versions of the ISW1 gene were constructed to follow Isw1p localization either in living (GFP-fusions) or in fixed cells (c-Myc-fusions). The whole set of available importin mutants was transformed with vectors carrying an intragenously-tagged ISW1 gene. In the strains where the importins Kap120p, Nmd5p, Sxm1p, Kap114p, Los1p, Msn5p, Kap123p and Pdr6 were deleted, the Isw1p-GFP fusion regulated by the ISW1 promoter was localized always in the nucleus. This suggests that none from these importins is required for nuclear localization of Isw1p. The localization of the Isw1p-GFP in the mtr10-1 strain differed from that observed in all the other analyzed importin mutants. Our results indicate that Mtr10p could be a good candidate for Isw1p importin. (en)
Title	Searching for a nuclear transportin of the chromatin-remodeling factor ISW1 in Saccharomyces cerevisiae Searching for a nuclear transportin of the chromatin-remodeling factor ISW1 in Saccharomyces cerevisiae (en) Vyhledávání jaderného transportinu faktoru ISW1 remodelujícího chromatin u Saccharomyces cerevisiae (cs)
skos:prefLabel	Searching for a nuclear transportin of the chromatin-remodeling factor ISW1 in Saccharomyces cerevisiae Searching for a nuclear transportin of the chromatin-remodeling factor ISW1 in Saccharomyces cerevisiae (en) Vyhledávání jaderného transportinu faktoru ISW1 remodelujícího chromatin u Saccharomyces cerevisiae (cs)
skos:notation	RIV/61388971:_____/04:00108117!RIV/2005/AV0/A53005/N
http://linked.open.../vavai/riv/strany	100
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA5020102), P(IAA5020409), Z(AV0Z5020903)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Strádalová, Vendula Malcová, Ivana Hašek, Jiří
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	585625
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/04:00108117
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	saccharomyces cerevisiae; isw1p (en)
http://linked.open.../riv/klicoveSlovo	saccharomyces cerevisiae isw1p
http://linked.open...ontrolniKodProRIV	[73B0A40BFEB8]
http://linked.open...v/mistoKonaniAkce	Prague
http://linked.open...i/riv/nazevZdroje	Symposium of the Society for Histochemistry /46./
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Yeast ISWI proteins and complexes - analysis of their subcellular localization and their role in the response to saline stress Role of chromatin remodelling factors interactions in nutrient-controlled gene expression in yeast
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Strádalová, Vendula Hašek, Jiří Janatová, Ivana
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2004-09-22 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Výzkum buněčné a molekulární biologie, genetiky, fyziologie a ekologie mikroorganizmů, mikrobní biotechnologie; studium imunitních procesů
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Neuveden

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