About: Construction of unmarked mutants in gene coding for Ser/Thr protein kinases and phosphoprotein phosphatase of Pseudomonas aeruginosa and analysis of theur properties     Goto   Sponge   NotDistinct   Permalink

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Description
  • Abychom zjistili význam jednotlivých proteinkinas a proteinfosfatas Pseudomonas aeruginosa byly připraveny mutanty v jednotlivých genech. Jednoduché mutanty byly připraveny metodou „gene replacement“ a rezistenční kazety byly posléze vyštěpeny pomocí Flp-FRT rekombinačního systému. Pomocí dvourozměrné elektroforézy byly porovnány fosforylační profily divokého typu a mutantů získané in vitro. (cs)
  • To investigate the importance of individual protein kinases and phosphatases in P. aeruginosa physiology we prepared the single and double mutants in the coresponding genes. Single mutants in protein kinase genes stk1, ppkB and protein phosphatase gene stp1 were prepared by the method of gene replacement with subsequent excision of antibiotic cassettes using Flp-FRT recombination system. Two-dimensional gel electrophoresis was used to analyze the patterns of phosphoproteins obtained in in vitro protein kinase assays with the cell-free lysates of wild type as well as all mutant strains.
  • To investigate the importance of individual protein kinases and phosphatases in P. aeruginosa physiology we prepared the single and double mutants in the coresponding genes. Single mutants in protein kinase genes stk1, ppkB and protein phosphatase gene stp1 were prepared by the method of gene replacement with subsequent excision of antibiotic cassettes using Flp-FRT recombination system. Two-dimensional gel electrophoresis was used to analyze the patterns of phosphoproteins obtained in in vitro protein kinase assays with the cell-free lysates of wild type as well as all mutant strains. (en)
Title
  • Construction of unmarked mutants in gene coding for Ser/Thr protein kinases and phosphoprotein phosphatase of Pseudomonas aeruginosa and analysis of theur properties
  • Konstrukce neoznačených mutantů v genech kódujících Ser/Thr proteinkinasy a fosfoproteinfosfatasu Pseudomonas aeruginosa a analýza jejich vlastností (cs)
  • Construction of unmarked mutants in gene coding for Ser/Thr protein kinases and phosphoprotein phosphatase of Pseudomonas aeruginosa and analysis of theur properties (en)
skos:prefLabel
  • Construction of unmarked mutants in gene coding for Ser/Thr protein kinases and phosphoprotein phosphatase of Pseudomonas aeruginosa and analysis of theur properties
  • Konstrukce neoznačených mutantů v genech kódujících Ser/Thr proteinkinasy a fosfoproteinfosfatasu Pseudomonas aeruginosa a analýza jejich vlastností (cs)
  • Construction of unmarked mutants in gene coding for Ser/Thr protein kinases and phosphoprotein phosphatase of Pseudomonas aeruginosa and analysis of theur properties (en)
skos:notation
  • RIV/61388971:_____/04:00100410!RIV/2005/AV0/A53005/N
http://linked.open.../vavai/riv/strany
  • 730
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z5020903)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 558649
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/04:00100410
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • flp-frt; dna (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [500934830BE6]
http://linked.open...v/mistoKonaniAkce
  • Praha
http://linked.open...i/riv/mistoVydani
  • Praha
http://linked.open...i/riv/nazevZdroje
  • European Congress of Clinical Microbiology and Infectious Diseases /14./
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Branny, Pavel
  • Hercík, Kamil
  • Lněnička, Petr
  • Nedvědová, Jana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Neuveden
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