About: Soapwort oxidoreductase is involved in trinitrotoluene detoxification

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rostlinné enzymy účastnící se degradace nitroaromatických látek dosud nebyly podrobněji biochemicky charakterizovány. Ze suspensní kultury mydlice lékařské (Saponaria officinalis L.) jsme izolovali novou rostlinnou oxidoreduktázu, která se účastní degradace trinitrotoluenu (TNT). Tento enzym katalyzuje první krok redukce nitroskupin TNT za přítomnosti NAD(P)H za anaerobních podmínek. Enzym je monomerní, má molekulovou hmotnost 29 kDa a vyskytuje se ve dvou isoformách (pI 4,8 a 5,1). podle spektrální a kativační analýzy enzym obsahuje flavinmononukleotid jako prostetickou skupinu. Ze strukturálních vlastností lze usuzovat na evoluční příbuznost k oxofytodienoát reduktáze. Pořadí aminokyselin N-terminálního konce vykazuje homologii s rodinou OYE (E.C. 1.6.99.1). (cs) Plant enzymes participating in degradation of nitroaromatic compounds have not been biochemically characterized in details so far. From suspension culture of soapwort (Saponaria officinalis L.) we isolated a novel plant oxidoreductase involved in degradation of trinitrotoluene (TNT). The enzyme catalyses first steps of reduction of TNT nitro groups in the presence of NAD(P)H under anaerobic conditions. The enzyme is monomeric with molecular mass 29 kDa, its two isoforms have pI 4.8 and 5.1. According to the spectral and activation analysis the enzyme contains flavinmono-nucleotide as a prosthetic group. The structure properties suggest an evolutional relationship to oxophytodienoate reductase. The N-terminal amino acid sequence shows homology to family of Old Yellow Enzyme (E.C. 1.6.99.1). Plant enzymes participating in degradation of nitroaromatic compounds have not been biochemically characterized in details so far. From suspension culture of soapwort (Saponaria officinalis L.) we isolated a novel plant oxidoreductase involved in degradation of trinitrotoluene (TNT). The enzyme catalyses first steps of reduction of TNT nitro groups in the presence of NAD(P)H under anaerobic conditions. The enzyme is monomeric with molecular mass 29 kDa, its two isoforms have pI 4.8 and 5.1. According to the spectral and activation analysis the enzyme contains flavinmono-nucleotide as a prosthetic group. The structure properties suggest an evolutional relationship to oxophytodienoate reductase. The N-terminal amino acid sequence shows homology to family of Old Yellow Enzyme (E.C. 1.6.99.1). (en)
Title	Soapwort oxidoreductase is involved in trinitrotoluene detoxification Soapwort oxidoreductase is involved in trinitrotoluene detoxification (en) Soapwort oxidoreductase is involved in trinitrotoluene detoxification (cs)
skos:prefLabel	Soapwort oxidoreductase is involved in trinitrotoluene detoxification Soapwort oxidoreductase is involved in trinitrotoluene detoxification (en) Soapwort oxidoreductase is involved in trinitrotoluene detoxification (cs)
skos:notation	RIV/61388963:_____/07:00305940!RIV08-AV0-61388963
http://linked.open.../vavai/riv/strany	367;371
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(1P05ME730), P(1P05OC042), V, Z(AV0Z40550506), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Nepovím, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	450622
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/07:00305940
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	flavoprotein; Old Yellow Enzyme; oxidoreductase (en)
http://linked.open.../riv/klicoveSlovo	oxidoreductase flavoprotein Old Yellow Enzyme
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[0C971CA95420]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Multidisciplinary study of the mechanism of phytoremediation of xenobiotics polluted soil and waters Study of basic processes influencing plant-environment interaction and its practical application
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Podlipná, Radka Vaněk, Tomáš Vágner, Martin Soudek, Petr Nepovím, Aleš
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0006-3134
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Soapwort oxidoreductase is involved in trinitrotoluene detoxification Soapwort oxidoreductase is involved in trinitrotoluene detoxification Soapwort oxidoreductase is involved in trinitrotoluene detoxification

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