About: Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fluorescence methods are versatile tools for obtaining dynamic and topological information about biomembranes because the molecular interactions taking place in lipid membranes frequently occur on the same timescale as fluorescence emission. The fluorescence intensity decay, in particular, is a powerful reporter of the molecular environment of a fluorophore. The fluorescence lifetime can be sensitive to the local polarity, hydration, viscosity, and/or presence of fluorescence quenchers/ energy acceptors within several nanometers of the vicinity of a fluorophore. Illustrative examples of how time-resolved fluorescence measurements can provide more valuable and detailed information about a system than the time-integrated (steady-state) approach will be presented in this review: 1), determination of membrane polarity and mobility using time-dependent spectral shifts; 2), identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidation of membrane leakage mechanisms from dye self-quenching assays; and 4), evaluation of nanodomain sizes by time-resolved Fo rster resonance energy transfer measurements. Fluorescence methods are versatile tools for obtaining dynamic and topological information about biomembranes because the molecular interactions taking place in lipid membranes frequently occur on the same timescale as fluorescence emission. The fluorescence intensity decay, in particular, is a powerful reporter of the molecular environment of a fluorophore. The fluorescence lifetime can be sensitive to the local polarity, hydration, viscosity, and/or presence of fluorescence quenchers/ energy acceptors within several nanometers of the vicinity of a fluorophore. Illustrative examples of how time-resolved fluorescence measurements can provide more valuable and detailed information about a system than the time-integrated (steady-state) approach will be presented in this review: 1), determination of membrane polarity and mobility using time-dependent spectral shifts; 2), identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidation of membrane leakage mechanisms from dye self-quenching assays; and 4), evaluation of nanodomain sizes by time-resolved Fo rster resonance energy transfer measurements. (en)
Title	Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State (en)
skos:prefLabel	Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State (en)
skos:notation	RIV/61388955:_____/14:00436040!RIV15-GA0-61388955
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GC14-03141J), P(LH13259)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Šachl, Radek Hof, Martin Jurkiewicz, Piotr Amaro, Mariana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	50589
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/14:00436040
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nanoparticles; reactivity; energetics (en)
http://linked.open.../riv/klicoveSlovo	energetics nanoparticles reactivity
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[03F2C74F97DC]
http://linked.open...i/riv/nazevZdroje	Biophysical Journal
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Studium vztahu struktury a funkce FGF2 oligomerů tvořících membránové póry technikami zaměřenými na sledování jedné molekuly Membrane interactions of proteins associated with alzheimer's disease and parkinson's disease implications for diseases' pathologies and therapeutic avenues
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	107
http://linked.open...iv/tvurceVysledku	Hof, Martin Jurkiewicz, Piotr Šachl, Radek Amaro, Mariana Coutinho, A. Prieto, M.
http://linked.open...ain/vavai/riv/wos	000346434200007
issn	0006-3495
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.bpj.2014.10.058

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