About: DNA Adduct Formation from Quaternary Benzo[c]phenanthridine Alkaloids Sanguinarine and Chelerythrine as Revealed by the 32P-postlabeling Technique.

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About: DNA Adduct Formation from Quaternary Benzo[c]phenanthridine Alkaloids Sanguinarine and Chelerythrine as Revealed by the 32P-postlabeling Technique. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Using the P-32-postlabeling assay, we investigated the ability of quaternary benzo[c]phenanthridine alkaloids, sanguinarine, chelerythrine and fagaronine, to form DNA adducts in vitro. Two enhanced versions of the assay (enrichment by nuclease P1 and 1-butanol extraction) were utilized in the study. Hepatic microsomes of rats pre-treated with beta-naphthoflavone or those of uninduced rats, used as metabolic activators, were incubated in the presence of calf thymus DNA and the alkaloids, with NADPH used as a cofactor. Under these conditions sanguinarine and chelerythrine, but not fagaronine, formed DNA adducts detectable by P-32-postlabeling. DNA adduct formation by both alkaloids was found to be concentration dependent. When analyzing different atomic and bond indices of the C-11-C-12 bond (ring B) in alkaloid molecules we found that fagaronine behaved differently from sanguinarine and chelerythrine. While sanguinarine and chelerythrine showed a preference. Using the P-32-postlabeling assay, we investigated the ability of quaternary benzo[c]phenanthridine alkaloids, sanguinarine, chelerythrine and fagaronine, to form DNA adducts in vitro. Two enhanced versions of the assay (enrichment by nuclease P1 and 1-butanol extraction) were utilized in the study. Hepatic microsomes of rats pre-treated with beta-naphthoflavone or those of uninduced rats, used as metabolic activators, were incubated in the presence of calf thymus DNA and the alkaloids, with NADPH used as a cofactor. Under these conditions sanguinarine and chelerythrine, but not fagaronine, formed DNA adducts detectable by P-32-postlabeling. DNA adduct formation by both alkaloids was found to be concentration dependent. When analyzing different atomic and bond indices of the C-11-C-12 bond (ring B) in alkaloid molecules we found that fagaronine behaved differently from sanguinarine and chelerythrine. While sanguinarine and chelerythrine showed a preference. (en)
Title	DNA Adduct Formation from Quaternary Benzo[c]phenanthridine Alkaloids Sanguinarine and Chelerythrine as Revealed by the 32P-postlabeling Technique. DNA Adduct Formation from Quaternary Benzo[c]phenanthridine Alkaloids Sanguinarine and Chelerythrine as Revealed by the 32P-postlabeling Technique. (en)
skos:prefLabel	DNA Adduct Formation from Quaternary Benzo[c]phenanthridine Alkaloids Sanguinarine and Chelerythrine as Revealed by the 32P-postlabeling Technique. DNA Adduct Formation from Quaternary Benzo[c]phenanthridine Alkaloids Sanguinarine and Chelerythrine as Revealed by the 32P-postlabeling Technique. (en)
skos:notation	RIV/61388955:_____/02:54020187!RIV/2003/AV0/A54003/N
http://linked.open.../vavai/riv/strany	231;242
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/00/0633), P(GA203/01/0996), P(NL5267), Z(AV0Z4040901), Z(MSM 113100001)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Hobza, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	643488
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/02:54020187
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA adduct formation; chelerythrine; sanquinarine (en)
http://linked.open.../riv/klicoveSlovo	sanquinarine chelerythrine DNA adduct formation
http://linked.open...odStatuVydavatele	IE - Irsko
http://linked.open...ontrolniKodProRIV	[428948C49478]
http://linked.open...i/riv/nazevZdroje	Chemico-Biological Interactions
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure, dynamics and function of DNA Study of molecular mechanism of anticancer effect of a chemotherapeutic, ellipticine Primary cultures of hepatocytes as a model for studies of cytoprotectivity of selected natural substances
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	140
http://linked.open...iv/tvurceVysledku	Hobza, Pavel Ulrichová, J. Frei, E. Šimánek, V. Stiborová, M.
http://linked.open...n/vavai/riv/zamer	Objasňování zákonitostí vztahů struktury a vlastností - zejména dynamiky a chemické reaktivity - látek a molekulárních systémů http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20113100001
issn	0009-2797
number of pages	12 (xsd:int)

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