About: One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins

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About: One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	N-terminal myristoylation of retroviral matrix proteins is essential for the targeting of the Gag polyproteins to the plasma membrane. To investigate the effect of the myristoylation on the structure and membrane binding ability of the matrix proteins, it is necessary to prepare their myristoylated forms. We present purification of myristoylated matrix proteins of the mouse mammary tumor virus and murine leukemia virus, two morphogenetically distinct retroviruses. The proteins were expressed in Escherichia coli coexpressing a yeast N-myristoyltransferase. This E. coli expression system yielded a mixture of myristoylated and nonmyristoylated matrix proteins. We established efficient one-step metal affinity purification that enabled to obtain pure myristoylated matrix proteins suitable for structural and functional studies. N-terminal myristoylation of retroviral matrix proteins is essential for the targeting of the Gag polyproteins to the plasma membrane. To investigate the effect of the myristoylation on the structure and membrane binding ability of the matrix proteins, it is necessary to prepare their myristoylated forms. We present purification of myristoylated matrix proteins of the mouse mammary tumor virus and murine leukemia virus, two morphogenetically distinct retroviruses. The proteins were expressed in Escherichia coli coexpressing a yeast N-myristoyltransferase. This E. coli expression system yielded a mixture of myristoylated and nonmyristoylated matrix proteins. We established efficient one-step metal affinity purification that enabled to obtain pure myristoylated matrix proteins suitable for structural and functional studies. (en)
Title	One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins (en)
skos:prefLabel	One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins (en)
skos:notation	RIV/60461373:22810/13:43897411!RIV15-MSM-22810___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA204/09/1388)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hrabal, Richard
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Rektorát
http://linked.open...dnocenehoVysledku	94030
http://linked.open...ai/riv/idVysledku	RIV/60461373:22810/13:43897411
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Retrovirus; N-myristoyltransferase; Myristoylation; Murine leukemia virus; Mouse mammary tumor virus; Matrix protein (en)
http://linked.open.../riv/klicoveSlovo	Matrix protein Myristoylation Retrovirus Mouse mammary tumor virus Murine leukemia virus N-myristoyltransferase
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[11C802833150]
http://linked.open...i/riv/nazevZdroje	Protein Expression and Purification
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterization of novel host cell protein interacting with protease of Mason-Pfizer monkey virus
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	92
http://linked.open...iv/tvurceVysledku	Pichová, Iva Hrabal, Richard Ruml, Tomáš Rumlová, Michaela Doležal, Michal Zábranský, Aleš
http://linked.open...ain/vavai/riv/wos	000326257000014
issn	1046-5928
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.pep.2013.09.003
http://localhost/t...ganizacniJednotka	22810

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