About: Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the experiments, the biological receptor is immobilized on the microchannel walls by a passive sorption. The blocking reagent 1 % BSA is used to cover empty regions on the walls. Then, aqueous solutions of ligands and detection reagents with a fluorescent marker are subsequently introduced in the channels. Finally, a solution of a detergent is used to break non-specific bonds among the biomolecules. The main advantage of the used experimental arrangement is that the reaction time of each reaction step in this experimental arrangement is not longer than few minutes due to the small characteristic dimensions. In the experiments, the biological receptor is immobilized on the microchannel walls by a passive sorption. The blocking reagent 1 % BSA is used to cover empty regions on the walls. Then, aqueous solutions of ligands and detection reagents with a fluorescent marker are subsequently introduced in the channels. Finally, a solution of a detergent is used to break non-specific bonds among the biomolecules. The main advantage of the used experimental arrangement is that the reaction time of each reaction step in this experimental arrangement is not longer than few minutes due to the small characteristic dimensions. (en)
Title	Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips (en)
skos:prefLabel	Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips (en)
skos:notation	RIV/60461373:22340/09:00021985!RIV10-MSM-22340___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD104/08/H055), P(KAN208240651), Z(MSM6046137306)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Slouka, Zdeněk Přibyl, Michal
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	318547
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/09:00021985
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	microfluidics; immunoassay; PDMS; fluorescence (en)
http://linked.open.../riv/klicoveSlovo	fluorescence microfluidics immunoassay PDMS
http://linked.open...ontrolniKodProRIV	[17664C6E898C]
http://linked.open...v/mistoKonaniAkce	Jablonec nad Nisou
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Sborník prací ze semináře Reakční a transportní jevy III
http://linked.open...in/vavai/riv/obor	CI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Transport and reaction processes in complex multiphase systems Study of interactions of biological macromolecules and nanolayers with a view to research of polymer microfluidic biosensors and therapeutic nanoparticles
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Slouka, Zdeněk Nebyla, Marek Přibyl, Michal Schrott, Walter
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open.../riv/zahajeniAkce	2009-06-10 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Reakčně-transportní chemicko-inženýrské systémy a jejich dynamické chování, modelování a řízení chemických a biotechnologických procesů
number of pages	9 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Vysoká škola chemicko-technologická v Praze
https://schema.org/isbn	978-80-7080-725-5
http://localhost/t...ganizacniJednotka	22340
is http://linked.open...avai/riv/vysledek of	Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips

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