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Description
  • Microfluidic protein chips are devices based on the complementary reaction between a ligand and a receptor. The characteristic size of the devices is in order of microns. This work is focused on study of bioaffinity assays in a polystyrene microchip. Two different arrangements for quantification of human immunoglobulins (hIgG) are described. In both cases, the assays are non-competitive and heterogeneous with protein A (PA) immobilized on polystyrene by a passive sorption as receptors. PA binds the Fc region of human immunoglobulins (ligand). In the one step assay, immobilized PA binds fluorescently labeled hIgG; in the sandwich assay, hIgG is bound as a primary ligand and then a fluorescently labeled anti-human goat IgG as the detection antibody. A mathematical model of the incubation phase of the one-step assay is focused on determination of the kinetic constants of heterogeneous PA-hIgG binding in the polystyrene microfluidic chip.
  • Microfluidic protein chips are devices based on the complementary reaction between a ligand and a receptor. The characteristic size of the devices is in order of microns. This work is focused on study of bioaffinity assays in a polystyrene microchip. Two different arrangements for quantification of human immunoglobulins (hIgG) are described. In both cases, the assays are non-competitive and heterogeneous with protein A (PA) immobilized on polystyrene by a passive sorption as receptors. PA binds the Fc region of human immunoglobulins (ligand). In the one step assay, immobilized PA binds fluorescently labeled hIgG; in the sandwich assay, hIgG is bound as a primary ligand and then a fluorescently labeled anti-human goat IgG as the detection antibody. A mathematical model of the incubation phase of the one-step assay is focused on determination of the kinetic constants of heterogeneous PA-hIgG binding in the polystyrene microfluidic chip. (en)
  • Byly studovány dvě experimentální uspořádání pro kvantifikaci lidských protilátek ze vzorku. Uspořádání byly nekompetitivní a heterogenní. Receptorem byl protein A a ligandem lidská protilátka. Detekce vzniklého komplexu byla prováděna fluorescenčně. Pomocí matematického modelu byly odhadnuty hodnoty kinetické a rovnovážné konstanty systémů Protein A - IgG. (cs)
Title
  • SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP
  • SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP (en)
  • Sendvičová bioanalýza v polystyrénovém mikročipu (cs)
skos:prefLabel
  • SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP
  • SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP (en)
  • Sendvičová bioanalýza v polystyrénovém mikročipu (cs)
skos:notation
  • RIV/60461373:22340/07:00018093!RIV08-AV0-22340___
http://linked.open.../vavai/riv/strany
  • 1-6
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  • P(GD104/03/H141), P(KAN208240651), Z(MSM6046137306)
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  • 448869
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  • RIV/60461373:22340/07:00018093
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http://linked.open.../riv/klicovaSlova
  • immunoassay; antibody; antigen; receptor; ligand; binding constant; human IgG; protein A; microdevice; immunosensor; biosensor; microchip; specific reaction rate (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [4F61848211B3]
http://linked.open...v/mistoKonaniAkce
  • Tatranské Matliare, Slovakia
http://linked.open...i/riv/mistoVydani
  • Bratislava
http://linked.open...i/riv/nazevZdroje
  • Proceedings of 34th International Conference of Slovak Society of Chemical Engineering, 21.-25.5.2007, Tatranské Matliare, Slovenská republika
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  • Přibyl, Michal
  • Marek, Miloš
  • Štěpánek, Jakub
  • Pecháčková, Lucie
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Slovenská společnost chemického inženýrství
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  • 978-80-227-2640-5
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  • 22340
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