| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Zvýšená biosorpce rtuti kvasinkami s MerR vystaveným na povrchu jako nový bakteriální celobuněčný biosensor (cs)
- The display of novel metal binding sites on the surface of the biosorbent represents potent tool to increase its binding capacity and improve selectivity. In this study, the 15.8 kDa transcriptional regulator MerR of mercury-inducible mer operon of Tn21 exhibiting high affinity and selectivity towards Hg2+, was displayed on the surface of Saccharomyces cerevisiae. To achieve this, merR was genetically fused with gene encoding C-terminal domain of -agglutinin which resulted in covalent attachment of the of the fusion protein on the cell wall glucan via glycosylphosphatidylinositol anchor. To evaluate the performance of such modified whole-cell biosorbent with specific regard to Hg2+, we constructed a new biosensor Escherichia coli strain, which utilizes kanamycine resistance gene as a reporter under the control of mer promoter. It allowed determination of Hg2+ in a range of 5 to 100 nM by simply monitoring the growth in the Hg2+/kanamycine-containing media. The effect of genetic engineering of S. cere
- The display of novel metal binding sites on the surface of the biosorbent represents potent tool to increase its binding capacity and improve selectivity. In this study, the 15.8 kDa transcriptional regulator MerR of mercury-inducible mer operon of Tn21 exhibiting high affinity and selectivity towards Hg2+, was displayed on the surface of Saccharomyces cerevisiae. To achieve this, merR was genetically fused with gene encoding C-terminal domain of -agglutinin which resulted in covalent attachment of the of the fusion protein on the cell wall glucan via glycosylphosphatidylinositol anchor. To evaluate the performance of such modified whole-cell biosorbent with specific regard to Hg2+, we constructed a new biosensor Escherichia coli strain, which utilizes kanamycine resistance gene as a reporter under the control of mer promoter. It allowed determination of Hg2+ in a range of 5 to 100 nM by simply monitoring the growth in the Hg2+/kanamycine-containing media. The effect of genetic engineering of S. cere (en)
|
| Title
| - Zvýšená biosorpce rtuti kvasinkami s MerR vystaveným na povrchu jako nový bakteriální celobuněčný biosensor (cs)
- Enhanced mercury biosorption by surface-engineered yeast displaying MerR inspected by new bacterial whole-cell biosensor.-poster
- Enhanced mercury biosorption by surface-engineered yeast displaying MerR inspected by new bacterial whole-cell biosensor.-poster (en)
|
| skos:prefLabel
| - Zvýšená biosorpce rtuti kvasinkami s MerR vystaveným na povrchu jako nový bakteriální celobuněčný biosensor (cs)
- Enhanced mercury biosorption by surface-engineered yeast displaying MerR inspected by new bacterial whole-cell biosensor.-poster
- Enhanced mercury biosorption by surface-engineered yeast displaying MerR inspected by new bacterial whole-cell biosensor.-poster (en)
|
| skos:notation
| - RIV/60461373:22330/05:00015542!RIV06-GA0-22330___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1M0520), P(GP203/03/P095)
|
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/05:00015542
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - biosorbent, Hg2+; Saccharomyces cerevisiae (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Kotrba, Pavel
- Ruml, Tomáš
- Vinopal, Stanislav
- Vopálenský, Pavel
|
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)