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rdf:type
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Description
| - Izolace a charakteristika proteinu p12 Mason-Pfizerova opičího viru (cs)
- The Mason#Pfizer monkey virus (M-PMV) Gag protein, precursor to the structural proteins of the infectious virion, assembles intoimmature capsid-like particles when expressed at high levels in bacterial cells. Similar capsid-like particles can be obtained by in vitroassembly using a high concentration of isolated Gag. M-PMV Gag contains a p12 protein that has no corresponding analogues in most otherretroviruses and has been suggested to contain an internal scaffold domain (ISD). We have expressed and purified p12 and the N- and Cterminalhalves (Np12 and Cp12) that are predicted to be structurally independent domains. The behavior of these proteins was analyzedusing chemical cross-linking, CD spectroscopy, and electron microscopy. The N-terminal half of p12 is largely a-helical although the Cterminalportion lacks any apparent ordered structure. Both p12 and Np12 form high-order oligomers in vitro and when expressed in E. coliproduce organized structures that are visible by electron microscopy. Interesti
- The Mason#Pfizer monkey virus (M-PMV) Gag protein, precursor to the structural proteins of the infectious virion, assembles intoimmature capsid-like particles when expressed at high levels in bacterial cells. Similar capsid-like particles can be obtained by in vitroassembly using a high concentration of isolated Gag. M-PMV Gag contains a p12 protein that has no corresponding analogues in most otherretroviruses and has been suggested to contain an internal scaffold domain (ISD). We have expressed and purified p12 and the N- and Cterminalhalves (Np12 and Cp12) that are predicted to be structurally independent domains. The behavior of these proteins was analyzedusing chemical cross-linking, CD spectroscopy, and electron microscopy. The N-terminal half of p12 is largely a-helical although the Cterminalportion lacks any apparent ordered structure. Both p12 and Np12 form high-order oligomers in vitro and when expressed in E. coliproduce organized structures that are visible by electron microscopy. Interesti (en)
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Title
| - Izolace a charakteristika proteinu p12 Mason-Pfizerova opičího viru (cs)
- Isolation and characterization of the Mason-Pfizer monkey virus p12 protein
- Isolation and characterization of the Mason-Pfizer monkey virus p12 protein (en)
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skos:prefLabel
| - Izolace a charakteristika proteinu p12 Mason-Pfizerova opičího viru (cs)
- Isolation and characterization of the Mason-Pfizer monkey virus p12 protein
- Isolation and characterization of the Mason-Pfizer monkey virus p12 protein (en)
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skos:notation
| - RIV/60461373:22330/04:00012824!RIV/2005/MSM/223305/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/04:00012824
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - SDS resistentní dimer;strukturní protein (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kodíček, Milan
- Sedláčková, Lenka
- Ruml, Tomáš
- Strohalm, Martin
- Knejzlík, Zdeněk
- Sakalian, Mike
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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