About: INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS.

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About: INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mason-Pfizer monkey virus assembles immature capsids in the cytoplasm and the temporal and spatial regulation of the maturation is critical, since the PR is not activated until immature capsids reach the cell membrane. Several evidences for a regulation of the activity of retroviral proteases by reversible oxidative modification of the cysteine residues were reported1,2. The structural and functional analysis of a mutant of M-PMV PR, in which two cysteine residues (Cys7, Cys106) were replaced by alanines confirmed, that this mutation decreases the stability, the proteolytic activity of PR, and the propensity of the monomers to form dimers3. Here we present results of a high resolution NMR structure of the inactive mutant (D26N) of the 12 kDa form of M-PMV PR with preserved cysteine residues. The data together with a biochemical characterization, MALDI-TOF analysis revealed the formation of an intramolecular disulphide bridge, which helps to stabilize the monomeric scaffold, and moves the chemical eq Mason-Pfizer monkey virus assembles immature capsids in the cytoplasm and the temporal and spatial regulation of the maturation is critical, since the PR is not activated until immature capsids reach the cell membrane. Several evidences for a regulation of the activity of retroviral proteases by reversible oxidative modification of the cysteine residues were reported1,2. The structural and functional analysis of a mutant of M-PMV PR, in which two cysteine residues (Cys7, Cys106) were replaced by alanines confirmed, that this mutation decreases the stability, the proteolytic activity of PR, and the propensity of the monomers to form dimers3. Here we present results of a high resolution NMR structure of the inactive mutant (D26N) of the 12 kDa form of M-PMV PR with preserved cysteine residues. The data together with a biochemical characterization, MALDI-TOF analysis revealed the formation of an intramolecular disulphide bridge, which helps to stabilize the monomeric scaffold, and moves the chemical eq (en) vliv cysteinových zbytků na aktivitu proteasy Mason Pfizerova opičího viru (cs)
Title	INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS. INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS. (en) vliv cysteinových zbytků na aktivitu proteasy Mason Pfizerova opičího viru (cs)
skos:prefLabel	INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS. INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS. (en) vliv cysteinových zbytků na aktivitu proteasy Mason Pfizerova opičího viru (cs)
skos:notation	RIV/60461373:22330/04:00012809!RIV/2005/MSM/223305/N
http://linked.open.../vavai/riv/strany	76
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LN00A079)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Zábranská, Helena Hrabal, Richard Ruml, Tomáš
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	567848
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/04:00012809
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cysteine residues;protease (en)
http://linked.open.../riv/klicoveSlovo	protease cysteine residues
http://linked.open...ontrolniKodProRIV	[4626669A6B5D]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	The Retrovirus Assembly meeting
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Integrated Genomics
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Pichová, Iva Hrabal, Richard Ruml, Tomáš Svatoš, Aleš Bauerová, Helena
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2004-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	JPM Tisk s. r. o.
https://schema.org/isbn	80-86313-13-1
http://localhost/t...ganizacniJednotka	22330

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