About: A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation

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About: A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Změna orientace helikální domény matrixového proteinu MPMV způsobená mutací jedné aminokyseliny (cs) Mason-Pfizer monkey virus (M-PMV) belongs to the family of betaretroviruses (formerly D-type retroviruses) which differ from lentiviruses (formerly C-type viruses) (e. g. HIV-1) in mechanism of immature capsid assembly. Matrix protein (MA), a virus structural protein, plays an essential role in different stages of retrovirus maturation. A single point mutation (R55F) in the sequence of the wt MA, however, changes morphogenesis of the virus and capsids are assembled at the plasma membrane instead of in the cytoplasm, similarly to the C-type retroviruses.We have focused on the study of three-dimensional structure and dynamics of both proteins (wt MA and R55F mutant) by NMR spectroscopy to reveal possible structural changes caused by the mutation (R55F). Although a structure of the wt MA has already been published as C-alpha trace, we decided to perform a new study of this protein to obtain a complete 3D structure. Doubly labelled (13C, 15N) proteins were prepared using recombinant techniques. Standard Mason-Pfizer monkey virus (M-PMV) belongs to the family of betaretroviruses (formerly D-type retroviruses) which differ from lentiviruses (formerly C-type viruses) (e. g. HIV-1) in mechanism of immature capsid assembly. Matrix protein (MA), a virus structural protein, plays an essential role in different stages of retrovirus maturation. A single point mutation (R55F) in the sequence of the wt MA, however, changes morphogenesis of the virus and capsids are assembled at the plasma membrane instead of in the cytoplasm, similarly to the C-type retroviruses.We have focused on the study of three-dimensional structure and dynamics of both proteins (wt MA and R55F mutant) by NMR spectroscopy to reveal possible structural changes caused by the mutation (R55F). Although a structure of the wt MA has already been published as C-alpha trace, we decided to perform a new study of this protein to obtain a complete 3D structure. Doubly labelled (13C, 15N) proteins were prepared using recombinant techniques. Standard (en)
Title	Změna orientace helikální domény matrixového proteinu MPMV způsobená mutací jedné aminokyseliny (cs) A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation (en)
skos:prefLabel	Změna orientace helikální domény matrixového proteinu MPMV způsobená mutací jedné aminokyseliny (cs) A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation (en)
skos:notation	RIV/60461373:22330/04:00012726!RIV/2005/GA0/223305/N
http://linked.open.../vavai/riv/strany	49
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/03/0490), P(LN00A079)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Lipov, Jan Hrabal, Richard Rumlová, Michaela Ruml, Tomáš Vlach, Jiří
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	553148
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/04:00012726
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	matrix protein;NMR (en)
http://linked.open.../riv/klicoveSlovo	matrix protein NMR
http://linked.open...ontrolniKodProRIV	[7DA1CA0E74AC]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	The retrovirus assembly meeting
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Integrated Genomics Study of relationships between structure and function of phenotypically relevant mutants of Mason-Pfizer monkey virus matrix protein
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Mareš, Jiří Vlach, Jiří Hrabal, Richard Lipov, Jan Ruml, Tomáš Rumlová, Michaela
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2004-10-02 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	JPM Tisk s. r. o.
https://schema.org/isbn	80-86313-13-1
http://localhost/t...ganizacniJednotka	22330

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