About: Tryptophan modification by 2-hydroxy-5-nitrobenzyl bromide studied by MALDI-TOF mass spectrometry

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Tryptophan modification by 2-hydroxy-5-nitrobenzyl bromide studied by MALDI-TOF mass spectrometry Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The reaction with 2-hydroxy-5-nitrobenzyl bromide (HNB) is a common covalent modification of tryptophan. It results in several products which were described by classical physico-chemical methods. For better understanding the structure of HNB-modified tryptophan, we synthesized a model peptide containing only one tryptophan, modified it by HNB and analyzed the product by MALDI-TOF mass spectrometry. Several multi-modified products (up to 5 HNB moieties per one tryptophan) were identified, surprisingly. An influence of HNB concentration and pH on a degree of modification was also analyzed. In addition, a splitting of modified tryptophan peaks in MALDI-TOF spectrum was detected; most probably, this effect is a common MALDI artifact of nitro-aromatic compounds which can make identification of HNB-modified tryptophan by MALDI-TOF MS very simple The reaction with 2-hydroxy-5-nitrobenzyl bromide (HNB) is a common covalent modification of tryptophan. It results in several products which were described by classical physico-chemical methods. For better understanding the structure of HNB-modified tryptophan, we synthesized a model peptide containing only one tryptophan, modified it by HNB and analyzed the product by MALDI-TOF mass spectrometry. Several multi-modified products (up to 5 HNB moieties per one tryptophan) were identified, surprisingly. An influence of HNB concentration and pH on a degree of modification was also analyzed. In addition, a splitting of modified tryptophan peaks in MALDI-TOF spectrum was detected; most probably, this effect is a common MALDI artifact of nitro-aromatic compounds which can make identification of HNB-modified tryptophan by MALDI-TOF MS very simple (en)
Title	Tryptophan modification by 2-hydroxy-5-nitrobenzyl bromide studied by MALDI-TOF mass spectrometry Tryptophan modification by 2-hydroxy-5-nitrobenzyl bromide studied by MALDI-TOF mass spectrometry (en)
skos:prefLabel	Tryptophan modification by 2-hydroxy-5-nitrobenzyl bromide studied by MALDI-TOF mass spectrometry Tryptophan modification by 2-hydroxy-5-nitrobenzyl bromide studied by MALDI-TOF mass spectrometry (en)
skos:notation	RIV/60461373:22330/03:00008980!RIV/2004/GA0/223304/N
http://linked.open.../vavai/riv/strany	811-816
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/02/0922)
http://linked.open...iv/cisloPeriodika	312
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Kodíček, Milan Strohalm, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	631632
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/03:00008980
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MALDI-TOF mass spectrometry, 2-hydroxy-5-nitrobenzyl bromide, Koshland reagent (en)
http://linked.open.../riv/klicoveSlovo	MALDI-TOF mass spectrometry 2-hydroxy-5-nitrobenzyl bromide Koshland reagent
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[C04BAE3142EB]
http://linked.open...i/riv/nazevZdroje	Biochemical and Biophysical Research Communications
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of MALDI-TOF (matrix assisted laser desorption ionisation - Time of Flight) mass spectrometry on the determination of protein conformation
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	2003
http://linked.open...iv/tvurceVysledku	Kodíček, Milan Pechar, Michal Strohalm, Martin
issn	0006-291X
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software