About: Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry

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About: Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Lysine ?-amino group reacts with citraconic anhydride forming a derivative which is stable on terms for trypsin cleavage. This modification changes the spectrum of peptides formed by the trypsin action; as the number of trypsin-sensitive sites is reduced, the peptides with higher molecular mass can survive in the digest. The various studies of proteins by MALDI-TOF mass spectrometry are often complicated by the low sequence coverage of the peptide chain. This paper demonstrates that the modification of proteins by citraconylation before trypsin cleavage represents a simple experimental technique which allows a significant increase of sequence coverage in MALDI-TOF mass spectrometry. This improvement is caused both by change of trypsin fragmentation pattern and by disturbance of the protein's native tertiary structure. Lysine ?-amino group reacts with citraconic anhydride forming a derivative which is stable on terms for trypsin cleavage. This modification changes the spectrum of peptides formed by the trypsin action; as the number of trypsin-sensitive sites is reduced, the peptides with higher molecular mass can survive in the digest. The various studies of proteins by MALDI-TOF mass spectrometry are often complicated by the low sequence coverage of the peptide chain. This paper demonstrates that the modification of proteins by citraconylation before trypsin cleavage represents a simple experimental technique which allows a significant increase of sequence coverage in MALDI-TOF mass spectrometry. This improvement is caused both by change of trypsin fragmentation pattern and by disturbance of the protein's native tertiary structure. (en)
Title	Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry (en)
skos:prefLabel	Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry (en)
skos:notation	RIV/60461373:22330/03:00008939!RIV/2004/GA0/223304/N
http://linked.open.../vavai/riv/strany	1091-1093
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/02/0922)
http://linked.open...iv/cisloPeriodika	305
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Kadlčík, Vojtěch Kodíček, Milan Strohalm, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	601327
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/03:00008939
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MALDI-TOF mass spectrometry, protein sequence coverage, citraconylation (en)
http://linked.open.../riv/klicoveSlovo	citraconylation protein sequence coverage MALDI-TOF mass spectrometry
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[657530158DF5]
http://linked.open...i/riv/nazevZdroje	Biochemical and Biophysical Research Communications
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of MALDI-TOF (matrix assisted laser desorption ionisation - Time of Flight) mass spectrometry on the determination of protein conformation
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	61
http://linked.open...iv/tvurceVysledku	Kodíček, Milan Strohalm, Martin Kadlčík, Vojtěch
issn	0006-291X
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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