About: Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS

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About: Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS Goto Sponge NotDistinct Permalink

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Description	Rapid and precise method for the detn. of 8-iso-prostaglandin F2, an essential marker of the oxidative stress, in exhaled breath condensate (EBC) was developed. The protocol consisted of stable isotope diln., immunosepn. combined with selective and sensitive LC-ESI-MS/MS operated in multiple reaction monitoring (MRM) mode. The imprecision of the developed method was below 8.8%, the parameter of mean inaccuracy was detd. as <9.6% (0-250 pg of 8-iso-prostaglandin F2/mL EBC). The limit of detection (LOD) was 1 pg/mL EBC and limit of quantification (LOQ) 5 pg/mL EBC. A significant difference in 8-iso-prostaglandin F2 content between the group of asbestosis patients and healthy volunteers was found. Rapid and precise method for the detn. of 8-iso-prostaglandin F2, an essential marker of the oxidative stress, in exhaled breath condensate (EBC) was developed. The protocol consisted of stable isotope diln., immunosepn. combined with selective and sensitive LC-ESI-MS/MS operated in multiple reaction monitoring (MRM) mode. The imprecision of the developed method was below 8.8%, the parameter of mean inaccuracy was detd. as <9.6% (0-250 pg of 8-iso-prostaglandin F2/mL EBC). The limit of detection (LOD) was 1 pg/mL EBC and limit of quantification (LOQ) 5 pg/mL EBC. A significant difference in 8-iso-prostaglandin F2 content between the group of asbestosis patients and healthy volunteers was found. (en) Detekce 8-iso-prostaglandinu F2 ve vydechovaném kondezátu vzduchu s použitím kombinace imunoseparace a LC-ESI-MS/MS (cs)
Title	Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS (en) Detekce 8-iso-prostaglandinu F2 ve vydechovaném kondezátu vzduchu s použitím kombinace imunoseparace a LC-ESI-MS/MS (cs)
skos:prefLabel	Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS (en) Detekce 8-iso-prostaglandinu F2 ve vydechovaném kondezátu vzduchu s použitím kombinace imunoseparace a LC-ESI-MS/MS (cs)
skos:notation	RIV/60461373:22310/08:00020246!RIV09-MZ0-22310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(NR8107), P(NR9338), Z(AV0Z50200510), Z(MSM 223100001), Z(MSM6046137301)
http://linked.open...iv/cisloPeriodika	867
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Kačer, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemické technologie
http://linked.open...dnocenehoVysledku	363038
http://linked.open...ai/riv/idVysledku	RIV/60461373:22310/08:00020246
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	8-iso-prostaglandin F2; exhaled breath condensate; immunoseparation (en)
http://linked.open.../riv/klicoveSlovo	exhaled breath condensate immunoseparation 8-iso-prostaglandin F2
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[82590367D38F]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Influence of individual factors on the levels of markers of oxidative stress in biological materials of subjects with exposure to asbestos and silica Ascertainment of oxidant stress in persons exposed to fibrogenic dusts
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Kačer, Petr Kuzma, Marek Lebedová, Jindřiška Pelclová, Daniela Syslová, Kamila Fenclová, Zuzana Klusackova, Pavlína
http://linked.open...ain/vavai/riv/wos	000256200400002
http://linked.open...n/vavai/riv/zamer	Catalytic processes in chemistry and chemical technology Microorganisms in Research and Biotechnology http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20223100001
issn	1570-0232
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	22310
is http://linked.open...avai/riv/vysledek of	Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS Determination of 8-iso-prostaglandin F2 in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS

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