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  • Bifidobacteria were enumerated and isolated from the faeces of lambs aged between 25 and 40 days using modified trypticase-phytone-yeast extract (TPY) agar supplemented with mupirocin and acetic acid. Nineteen strains were characterised and divided into 6 groups according their fermentation characteristics obtained by API 50 CHL and RAPID ID 32 tests and by randomly amplified polymorphic DNA (RAPD) profiles. One representative strain of each group was identified by sequencing by 16S rRNA and hsp60 genes sequencing as B. animalis ssp. animalis, B. choerinum, B. pseudolongum ssp. globosum, B. pseudocatenulatum. Examination of the DNA fingerprinting showed a genetic diversity between strains of B. animalis ssp. animalis and B. choerinum. Our result shows that some bifidobacterial species should not be host-specific or have a broad host spectrum. As bifidobacteria were determined as numerous bacterial group in the intestine of lambs on a milk diet (8.32 +/- 0.71 log CFU/g, mean count +/- S.D., n = 30), t
  • Bifidobacteria were enumerated and isolated from the faeces of lambs aged between 25 and 40 days using modified trypticase-phytone-yeast extract (TPY) agar supplemented with mupirocin and acetic acid. Nineteen strains were characterised and divided into 6 groups according their fermentation characteristics obtained by API 50 CHL and RAPID ID 32 tests and by randomly amplified polymorphic DNA (RAPD) profiles. One representative strain of each group was identified by sequencing by 16S rRNA and hsp60 genes sequencing as B. animalis ssp. animalis, B. choerinum, B. pseudolongum ssp. globosum, B. pseudocatenulatum. Examination of the DNA fingerprinting showed a genetic diversity between strains of B. animalis ssp. animalis and B. choerinum. Our result shows that some bifidobacterial species should not be host-specific or have a broad host spectrum. As bifidobacteria were determined as numerous bacterial group in the intestine of lambs on a milk diet (8.32 +/- 0.71 log CFU/g, mean count +/- S.D., n = 30), t (en)
Title
  • Identification of bifidobacteria from intestinal tract of lambs.
  • Identification of bifidobacteria from intestinal tract of lambs. (en)
skos:prefLabel
  • Identification of bifidobacteria from intestinal tract of lambs.
  • Identification of bifidobacteria from intestinal tract of lambs. (en)
skos:notation
  • RIV/60460709:41210/12:56194!RIV14-GA0-41210___
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  • P(GA523/08/1091), P(GD525/08/H060), Z(MSM6046070901)
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  • 1-3
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  • 140279
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  • RIV/60460709:41210/12:56194
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  • differentiation, probiotics, calves (en)
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  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [27EFB9238C68]
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  • Small Ruminant Research
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  • 105
http://linked.open...iv/tvurceVysledku
  • Killer, Jiří
  • Vlková, Eva
  • Rada, Vojtěch
  • Ročková, Šárka
  • Bunešová, Věra
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  • WOS:00030510090
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  • 0921-4488
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  • 41210
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