About: A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The objective of this work was to create a fast and sensitive method of detecting Paenibacillus larvae from beehive debris based on PCR that does not require long-lasting cultivation steps. Various methods of extracting spores from beehive debris were compared: the original method of extraction of spores into toluene, and alternative spore extraction methods into Tween 80, into water, into isopropanol and into 95% ethanol. Isolation of DNA from various spore extractions was evaluated too. Best results were provided by isolation of DNA using the QIAamp DNA Mini Kit, without heat treatment. DNA of spores was detected by PCR from 0.25 g of beeswax debris, with the detected titer of 105 in 1 g according to the cultivation tests. The objective of this work was to create a fast and sensitive method of detecting Paenibacillus larvae from beehive debris based on PCR that does not require long-lasting cultivation steps. Various methods of extracting spores from beehive debris were compared: the original method of extraction of spores into toluene, and alternative spore extraction methods into Tween 80, into water, into isopropanol and into 95% ethanol. Isolation of DNA from various spore extractions was evaluated too. Best results were provided by isolation of DNA using the QIAamp DNA Mini Kit, without heat treatment. DNA of spores was detected by PCR from 0.25 g of beeswax debris, with the detected titer of 105 in 1 g according to the cultivation tests. (en)
Title	A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris (en)
skos:prefLabel	A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris (en)
skos:notation	RIV/60460709:41210/09:31056!RIV10-MSM-41210___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021620828), Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika	0
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Titěra, Dalibor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	301495
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/09:31056
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Honeybee, Paenibacillus larvae, American foulbrood (AFB), Hive debris (en)
http://linked.open.../riv/klicoveSlovo	American foulbrood (AFB) Hive debris Paenibacillus larvae Honeybee
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6EA342734D56]
http://linked.open...i/riv/nazevZdroje	Veterinary Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	139
http://linked.open...iv/tvurceVysledku	Stopka, Pavel Titěra, Dalibor Ryba, Štěpán Haklová, Marcela
http://linked.open...n/vavai/riv/zamer	Ekologické procesy v evoluci modelových skupin organismů Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje.
issn	0378-1135
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	41210
is http://linked.open...avai/riv/vysledek of	A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software